Description

N3-PEG24-CH2CH2COOH is a high-purity, heterobifunctional polyethylene glycol (PEG) derivative featuring an azide (N3) group and a carboxylic acid (COOH) terminus, separated by a flexible 24-unit PEG spacer and a short ethylene linker. This molecular architecture is critical for creating stable, hydrophilic conjugates and spacers in advanced bioconjugation and materials science applications. It is essential for researchers and manufacturers in pharmaceuticals, diagnostics, and nanotechnology who require precise, water-soluble linkers for coupling biomolecules via click chemistry and carbodiimide-mediated reactions.

Application

• Bioconjugation & Drug Delivery: Serves as a versatile linker for attaching targeting ligands, dyes, or drugs to nanoparticles, proteins, or antibodies via copper-free click chemistry (azide-alkyne cycloaddition) and subsequent EDC/NHS coupling.
• PEGylation Reagent: Used to introduce PEG chains onto therapeutic peptides, proteins, or small molecules to enhance solubility, prolong circulation half-life, and reduce immunogenicity.
• Hydrogel & Biomaterial Synthesis: Acts as a crosslinking agent or functional monomer for creating biocompatible, tunable hydrogels for tissue engineering and controlled release systems.
• Diagnostic Assay Development: Facilitates the stable immobilization of capture antibodies or antigens on sensor surfaces and microplates for ELISA and biosensor applications.
• Surface Functionalization: Enables the modification of gold nanoparticles, quantum dots, and other material surfaces with carboxylic acid groups for further biomolecule attachment.
• Click Chemistry Scaffold: Provides a defined-length, hydrophilic spacer for synthesizing complex molecular architectures through sequential conjugation steps.

Basic Information

Product Name	N3-PEG24-CH2CH2COOH
CAS No.	2195075-62-6
Molecular Formula	C55H109N3O27
Molecular Weight	~1244.5 g/mol
Synonyms	Azido-PEG24-acid; N3-PEG24-acetic acid; 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]acetic acid; Azido-PEG24-COOH; PEG24-Azide-Acid; Heterobifunctional PEG Azide Acid
EINECS	Contact for details

Quality Control

Every batch of N3-PEG24-CH2CH2COOH is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure identity, purity, and consistency. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay by NMR/HPLC, functional group quantification, and impurity profiles. Our production adheres to cGMP guidelines where applicable, supporting use in pre-clinical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white waxy solid or viscous liquid
Identification (NMR)	Complies with structure
Identification (IR)	Complies (Azide & C=O peaks)
Purity (HPLC)	≥ 95%
Assay (Functional Group)	≥ 90% (Azide & COOH)
Water Content (KF)	≤ 1.0%
Molecular Weight (SEC/MS)	Within ± 5% of theoretical
Solubility	Clear, colorless solution in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.