Description

Sulfo Cy7 Maleimide is a near-infrared (NIR) fluorescent dye functionalized with a maleimide group for site-specific conjugation to thiols (sulfhydryl groups) in biomolecules. This compound is essential for creating highly sensitive and stable fluorescent probes and labels for advanced bioimaging and diagnostic applications. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, in vivo imaging, flow cytometry, and the development of antibody-drug conjugates (ADCs) and other therapeutic biologics.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific labeling of engineered cysteine residues on monoclonal antibodies for the creation of homogeneous, therapeutically relevant fluorescent ADCs.
• In Vivo Imaging Probes: Conjugation to peptides, proteins, or nanoparticles to generate NIR fluorescent tracers for deep-tissue imaging in preclinical animal studies.
• Protein Labeling & Tracking: Covalent attachment to thiol-containing proteins for monitoring protein-protein interactions, localization, and turnover via fluorescence-based assays.
• Flow Cytometry & Cell Analysis: Labeling of cell surface thiols or thiolated antibodies for high-sensitivity detection in multicolor flow cytometry panels.
• Biomarker Detection Assays: Development of fluorescent immunosensors and diagnostic kits utilizing the bright, stable fluorescence of the sulfo-Cy7 dye.
• Nanoparticle Functionalization: Conjugation to thiolated quantum dots, gold nanoparticles, or liposomes to create targeted imaging agents.

Basic Information

Product Name	Sulfo Cy7 Maleimide
CAS No.	2183440-60-8
Molecular Formula	C44H47KN2O13S3
Molecular Weight	995.23 g/mol
Synonyms	Sulfo-Cyanine7 maleimide; Cy7 Sulfo Maleimide; Sulfo Cyanine 7 Maleimide; 2-[(1E,3E)-5-[2-[2-(2,5-Dioxo-1-pyrrolidinyl)oxy]-2-oxoethyl]-1,3,3-trimethyl-5-sulfo-3H-indol-1-ium-2-yl]-1,3-propadienyl]-1-ethyl-3,3-dimethyl-3H-indolium; Maleimide-functionalized Sulfo-Cy7; NIR dye maleimide; Thiol-reactive Sulfo Cy7 dye
EINECS	Contact for details

Quality Control

Our Sulfo Cy7 Maleimide is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and absorbance/fluorescence spectroscopy for performance validation. A Certificate of Analysis (COA) detailing batch-specific results is provided to ensure traceability and compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue-green solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	747 - 753 nm
Emission Maximum (λem)	773 - 779 nm
Extinction Coefficient (ε)	≥ 200,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.