Description

Cy5.5 Azide is a near-infrared fluorescent dye functionalized with a reactive azide group for bioorthogonal click chemistry applications. This compound is essential for researchers and developers requiring high-sensitivity labeling and detection in complex biological systems. It is primarily used by professionals in the pharmaceutical, biotechnology, and life sciences industries for applications such as bioimaging, biomarker discovery, and the development of diagnostic assays.

Application

• Bioorthogonal Labeling: Selective conjugation to alkyne-modified biomolecules (proteins, antibodies, nucleic acids) via copper-free click chemistry for minimal cellular toxicity.
• In Vivo and In Vitro Imaging: High-sensitivity near-infrared (NIR) fluorescence imaging for deep-tissue penetration and reduced background autofluorescence in animal studies.
• Diagnostic Probe Development: Synthesis of targeted fluorescent probes for the detection and visualization of specific cellular targets or disease biomarkers.
• Flow Cytometry and Microscopy: Cell surface and intracellular labeling for analysis by flow cytometry, confocal microscopy, and super-resolution imaging techniques.
• Drug Delivery Tracking: Conjugation to drug carriers or therapeutic agents to monitor biodistribution, pharmacokinetics, and target engagement in preclinical research.
• Material Science Functionalization: Surface modification of nanoparticles, polymers, and other materials to create NIR-fluorescent platforms for sensing and delivery.

Basic Information

Product Name	Cy5.5 Azide
CAS No.	2183440-52-8
Molecular Formula	C44H51N6O6S2+
Molecular Weight	832.04 g/mol
Synonyms	Sulfo-Cyanine5.5 azide; Cyanine5.5 N3; NIR-Azide Dye; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-5-((1E,3E)-3-(3-(3-azidopropyl)-2,3-dihydro-1H-benzo[e]indol-1-yl)allylidene)-4-oxocyclopent-2-en-1-ylidene)ethylidene)-1H-benzo[e]indol-3-ium; 2-[2-[2-chloro-3-[(1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-1-propylindolium azide
EINECS	Contact for details

Quality Control

Our Cy5.5 Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~683 nm
Emission Maximum (λem)	~703 nm
Extinction Coefficient (ε)	≥ 190,000 cm-1M-1
Solubility	Soluble in DMSO, DMF, methanol
Functional Test (Click Reaction)	≥ 90% conjugation efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.