Description

Sulfo-Cy5.5 Amine is a near-infrared (NIR) fluorescent dye derivative functionalized with a reactive amine group and water-solubilizing sulfonate moieties. This compound matters for researchers and developers requiring high-performance, stable labeling for biomolecules in aqueous environments without compromising fluorescence intensity. Professionals in bioconjugation, in vivo imaging, and diagnostic assay development need it to create targeted probes with enhanced signal-to-noise ratios.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of antibodies, proteins, peptides, and oligonucleotides via amine-reactive chemistry.
• In Vivo and Whole-Animal Imaging: Creation of NIR fluorescent probes for deep-tissue imaging due to minimal tissue autofluorescence and absorption in the Cy5.5 emission range.
• Diagnostic Assay Development: Used as a detection label in fluorescence immunoassays (FLIA), flow cytometry, and microarray applications.
• Cell Labeling and Tracking: Long-term cell tracing and migration studies in biological research.
• Drug Delivery Research: Conjugation to nanoparticles or drug carriers for visualizing biodistribution and pharmacokinetics.
• Biomaterial Science: Fluorescent tagging of hydrogels, polymers, and other materials to monitor degradation or cellular interaction.

Basic Information

Product Name	Sulfo-Cy5.5 Amine
CAS No.	2183440-46-0
Molecular Formula	C44H55KN4O10S3 (Potassium salt form typical)
Molecular Weight	~983.3 g/mol
Synonyms	Sulfo-Cyanine5.5 amine; Sulfo-Cy5.5 NHS ester precursor; Cy5.5 amine, sulfonated; Water-soluble Cy5.5 amine; NIR dye amine derivative; 2-((1E,3E)-5-((E)-2-((1E,3E)-3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5-(1,1-dimethyl-3-sulfonatopropyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)ethylidene)-2-((E)-2-((E)-3-(1,1-dimethyl-3-sulfonatopropyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)ethylidene)cyclopentan-1-ylidene)ethylidene)-1,1-dimethyl-3-sulfonatopropyl-1H-benzo[e]indol-3-ium; Sulfo-Cy5.5 SE intermediate
EINECS	Contact for details

Quality Control

Our Sulfo-Cy5.5 Amine is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for absorbance/fluorescence verification. Certificates of Analysis (COA) detailing batch-specific results are available upon request to ensure compliance with your research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~683 nm
Emission (λmax)	~703 nm
Extinction Coefficient (ε)	≥ 190,000 cm⁻¹M⁻¹
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.