Description

n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester) is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and two NHS ester termini. This compound is engineered for high-efficiency bioconjugation, enabling the stable and site-specific coupling of biomolecules through click chemistry and amine-reactive linkages. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science, facilitating the creation of novel drug conjugates, diagnostic probes, and functionalized surfaces.

Application

• ADC (Antibody-Drug Conjugate) Linker Synthesis: For constructing stable, cleavable, or non-cleavable linkers between monoclonal antibodies and cytotoxic payloads.
• PEGylation of Peptides and Proteins: To introduce PEG chains for improving pharmacokinetic properties, solubility, and stability of therapeutic biologics.
• Surface Functionalization and Biomaterial Engineering: For modifying nanoparticles, biosensors, or hydrogel matrices to create bioactive surfaces for cell culture or diagnostics.
• Click Chemistry-Based Bioconjugation: Utilizes the azide group for copper-catalyzed (CuAAC) or strain-promoted (SPAAC) cycloaddition with alkynes.
• Crosslinking Agent for Protein-Protein or Protein-Ligand Studies: Used in structural biology and proteomics to capture and stabilize molecular interactions.
• Synthesis of Fluorescent or Imaging Probes: Conjugation of dyes, chelators, or other reporter molecules to targeting agents like antibodies or peptides.
• Polymer and Dendrimer Modification: As a branching or functionalization unit in the synthesis of complex polymeric architectures.

Basic Information

Product Name	n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester)
CAS No.	2182601-77-8
Molecular Formula	C49H80N6O23
Molecular Weight	1121.2 g/mol
Synonyms	Azido-PEG4-tris-NHS ester; N3-PEG4-tris-NHS; 1-Azido-3,6,9,12-tetraoxapentadecane-15-oic acid, 15-[(2,5-dioxo-1-pyrrolidinyl)oxy]-2,5,8,11-tetraoxatetradecan-14-yl ester; NHS-PEG4-Azide; Azido-PEG4-NHS ester crosslinker; Heterotrifunctional PEG crosslinker, Azide-NHS2; PEG-based tri-functional linker
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester) is manufactured under strict quality systems. Each batch undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and MS for identity verification, to ensure consistency and performance for critical research and GMP-grade applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the NHS ester groups are susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms (M+Na+ or M+H+)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 85% of theoretical
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.