Description

Cyanine5 Dbco CAS NO 2182601-71-2 is a near-infrared fluorescent dye functionalized with a dibenzocyclooctyne (DBCO) group for bioorthogonal click chemistry. This compound is critical for enabling highly specific, copper-free labeling and conjugation of biomolecules such as proteins, antibodies, and oligonucleotides without interfering with biological processes. It is essential for researchers and developers in bioconjugation, in vivo imaging, diagnostic assay development, and pharmaceutical R&D who require sensitive and stable fluorescent probes.

Application

• Bioorthogonal Labeling: Copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) for tagging azide-modified biomolecules in live cells and complex biological systems.
• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or reporter molecules to monoclonal antibodies for targeted therapy and diagnostics.
• In Vivo and In Vitro Imaging: Fluorescent labeling for near-infrared (NIR) fluorescence microscopy, flow cytometry, and small animal imaging due to its deep tissue penetration and low background.
• Diagnostic Assay Reagents: Development of fluorescent probes and detection reagents for ELISA, lateral flow assays, and other immunoassay platforms.
• Nucleic Acid Labeling: Conjugation to azide-modified oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Protein and Peptide Tagging: Site-specific modification of recombinant proteins and peptides for structural studies, tracking, and interaction analysis.
• Polymer and Material Science: Functionalization of azide-containing polymers, nanoparticles, and surfaces for creating smart materials and biosensors.

Basic Information

Item	Details
Product Name	Cyanine5 Dbco
CAS No.	2182601-71-2
Molecular Formula	C44H45N4O3+
Molecular Weight	677.86 g/mol
Synonyms	DBCO-Cy5; Cy5 DBCO; Sulfo-Cyanine5 DBCO (for sulfonated forms); DBCO-Cyanine5; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((E)-2-((E)-3-((E)-2-(1-(2-(2-(2-((1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethoxy)ethoxy)ethoxy)ethyl)-1H-1,2,3-triazol-4-yl)vinyl)-5,5-dimethylcyclohex-2-en-1-ylidene)ethylidene)indolin-1-ium; DBCO-PEGn-Cy5 (for PEGylated derivatives); Near-IR DBCO Dye
EINECS	Contact for details

Quality Control

Our Cyanine5 Dbco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to green solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Solubility	Soluble in DMSO, DMF, methanol
Water Content (KF)	< 5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.