Description

N3-Peg5-Ch2Co2-Nhs is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide (N3) group and an N-hydroxysuccinimide (NHS) ester, separated by a flexible pentameric ethylene glycol spacer. This compound is essential for creating stable, site-specific conjugations in aqueous environments, particularly in bioconjugation and materials science. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for labeling, immobilization, and the development of advanced drug delivery systems.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, peptides, and other biomolecules via click chemistry (azide-alkyne cycloaddition) and NHS-ester coupling to amines.
• Drug Delivery System Development: Construction of PEGylated drug conjugates and linkers for controlled-release formulations, enhancing solubility and pharmacokinetics.
• Surface Functionalization: Immobilization of biomolecules onto nanoparticles, biosensors, and microarray slides to create bioactive surfaces.
• Polymer and Hydrogel Synthesis: Serving as a versatile crosslinking agent for creating functionalized, biocompatible polymeric networks.
• Diagnostic Reagent Preparation: Conjugation of detection probes (e.g., fluorophores, enzymes) to targeting ligands for ELISA, flow cytometry, and in vitro diagnostics (IVD).
• ADC (Antibody-Drug Conjugate) Linker: As a component in the design of cleavable or non-cleavable linkers connecting cytotoxic payloads to monoclonal antibodies.
• Materials Science Research: Modification of material interfaces to introduce bio-recognition elements or alter surface properties.

Basic Information

Product Name	N3-Peg5-Ch2Co2-Nhs
CAS No.	2144777-77-3
Molecular Formula	C24H40N4O12
Molecular Weight	576.60 g/mol
Synonyms	Azido-PEG5-NHS ester; 5-Azido-3,6,9,12,15-pentaoxaheptadecan-1-oic acid 2,5-dioxopyrrolidin-1-yl ester; N3-PEG5-NHS; NHS-PEG5-Azide; Azido-PEG5-NHS; 1-[2-[2-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]acetyloxy]-2,5-pyrrolidinedione; Azido-Pentaethylene glycol-NHS ester
EINECS	Contact for details

Quality Control

Our N3-Peg5-Ch2Co2-Nhs is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group integrity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the NHS ester is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (NHS Ester)	≥ 90%
Solubility	Soluble in DMF, DMSO, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.