Description

Dbco-Peg5-Nhs Ester is a heterobifunctional crosslinking reagent designed for bioorthogonal conjugation. This compound is critical for creating stable, site-specific linkages between biomolecules and various probes or surfaces via strain-promoted azide-alkyne cycloaddition (SPAAC) and amine-reactive chemistry. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, proteomics, and the development of antibody-drug conjugates (ADCs) and diagnostic assays.

Application

• Bioconjugation and ADC Development: Site-specific labeling of antibodies, proteins, and peptides for therapeutic and diagnostic applications.
• Proteomics Research: Immobilization of biomolecules or creation of crosslinked complexes for mass spectrometry and interaction studies.
• Diagnostic Assay Fabrication: Conjugation of detection molecules (e.g., enzymes, fluorophores) to solid supports or antibodies for ELISA and lateral flow tests.
• Polymer and Material Science: Functionalization of nanoparticles, hydrogels, and surfaces with bioactive ligands for targeted delivery and sensing.
• Chemical Biology Tool Development: Synthesis of activity-based probes and other reagents for studying biological processes in live cells.

Basic Information

Product Name	Dbco-Peg5-Nhs Ester
CAS No.	2144395-59-3
Molecular Formula	C39H55N3O12
Molecular Weight	757.88 g/mol
Synonyms	DBCO-PEG5-NHS Ester; Dibenzocyclooctyne-PEG5-N-hydroxysuccinimide Ester; DBCO-PEG5-NHS; DBCO-PEG5-NHS Ester; 6-((6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)oxy)-6-oxohexyl 1-(1-oxo-6-((3aR,6S,6aR)-6-(prop-2-yn-1-yl)-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)hexyl)-1,4,7,10,13-pentaoxa-16-azacyclooctadecan-16-oate; Azadibenzocyclooctyne-PEG5-NHS Ester; ADIBO-PEG5-NHS Ester
EINECS	Contact for details

Quality Control

Our Dbco-Peg5-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high purity and consistent performance for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional integrity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.