Description

Maleimide-NH-PEG12-CH2CH2COOH-PFP Ester is a heterobifunctional, amine-reactive crosslinking reagent featuring a long, hydrophilic polyethylene glycol (PEG) spacer. This compound is engineered for the site-specific conjugation of biomolecules, offering superior aqueous solubility and reduced steric hindrance compared to shorter linkers. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced diagnostics, enabling the creation of stable, functionalized constructs.

Application

• Antibody-Drug Conjugate (ADC) Linker-Payload Synthesis: For the controlled attachment of cytotoxic drugs to monoclonal antibodies via amine groups.
• Protein and Peptide Labeling: Conjugation of fluorescent dyes, biotin, or other probes to lysine residues for imaging and detection assays.
• Surface Functionalization: Modification of amine-coated nanoparticles, beads, or biosensor surfaces to introduce maleimide groups for subsequent thiol coupling.
• Polymer Chemistry: Serving as a macromolecular building block for synthesizing functionalized PEG-based hydrogels or smart materials.
• Diagnostic Reagent Development: Creating stable immunoconjugates for ELISA, lateral flow assays, and other diagnostic platforms.
• Enzyme Immobilization: Covalent attachment of enzymes to solid supports while maintaining activity through a flexible PEG tether.

Basic Information

Product Name	Maleimide-NH-PEG12-CH2CH2COOH-PFP Ester
CAS No.	2136296-33-6
Molecular Formula	C68H108F5N3O30
Molecular Weight	~1552.6 g/mol
Synonyms	Maleimide-PEG12-PFP Ester; PFP-PEG12-Maleimide; 4-Maleimidobutyric acid-PEG12-PFP ester; NHS-PEG12-Maleimide (PFP ester variant); Fmoc-NH-PEG12-COOH-PFP Ester (precursor); Heterobifunctional PEG linker, amine and thiol reactive; Pentafluorophenyl 3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatetracontan-39-oate 40-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl); Amine-reactive PEG-Maleimide crosslinker.
EINECS	Contact for details

Quality Control

Every batch of Maleimide-NH-PEG12-CH2CH2COOH-PFP Ester is manufactured under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to verify identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional group activity is provided with each shipment to ensure it meets the exacting standards for bioconjugation and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the pentafluorophenyl ester moiety is susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity (Maleimide)	≥ 90% by thiol titration
Functional Activity (PFP Ester)	≥ 90% by amine coupling assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.