Description

Sulfo-Cy5-Tco CAS NO 2129525-69-3 is a water-soluble, near-infrared fluorescent dye functionalized with a trans-cyclooctene (TCO) group for bioorthogonal click chemistry. This compound is essential for advanced bioconjugation and labeling applications where minimal background fluorescence and high stability in aqueous environments are critical. It is primarily needed by researchers and manufacturers in the fields of bioimaging, diagnostics, and pharmaceutical development for creating targeted probes and antibody-drug conjugates (ADCs).

Application

• Bioorthogonal Labeling in Live-Cell Imaging: Utilized for real-time, high-contrast visualization of cellular processes via strain-promoted azide-alkyne cycloaddition (SPAAC) with tetrazine-modified targets.
• Antibody-Drug Conjugate (ADC) Development: Serves as a fluorescent payload or linker-dye component for tracking conjugate distribution, stability, and cellular uptake.
• In Vivo Fluorescence Imaging: Enables deep-tissue imaging in animal models due to its near-infrared emission, minimizing tissue autofluorescence and light scattering.
• Diagnostic Assay Reagents: Used in the development of fluorescent lateral flow assays, ELISA, and other diagnostic platforms requiring sensitive and stable signal generation.
• Protein and Biomolecule Conjugation: Facilitates the site-specific labeling of proteins, peptides, and oligonucleotides for functional studies and analytical detection.
• Polymer and Nanoparticle Functionalization: Incorporated into biomaterials and drug delivery vehicles for traceable targeting and biodistribution studies.

Basic Information

Product Name	Sulfo-Cy5-Tco
CAS No.	2129525-69-3
Molecular Formula	C45H51KN4O10S2
Molecular Weight	911.22 g/mol
Synonyms	Sulfo-Cyanine5-trans-cyclooctene; Sulfo-Cy5-TCO; Cy5 TCO sulfonated; 2-((1E,3E,5E)-5-(1-(5-((E)-3-(3-((E)-trans-cyclooct-2-en-1-yloxy)prop-1-en-1-yl)thiophen-2-yl)-3,3-dimethyl-5-sulfo-1-(5-sulfonatopentyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-1-(5-sulfonatopentyl)-3H-indol-1-ium-5-yl)ethanesulfonate (Potassium Salt); Sulfo-Cy5 TCO dye; Water-soluble Cy5-TCO; NIR TCO dye; Bioorthogonal Cy5 dye
EINECS	Contact for details

Quality Control

Our Sulfo-Cy5-Tco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic confirmation (UV-Vis, NMR, MS), to ensure identity, strength, and performance. Certificates of Analysis (COA) with detailed chromatographic and spectral data are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV-Vis)	λmax matches reference spectrum (typically ~646 nm in aqueous buffer)
Purity (HPLC)	≥95.0% (Area percent at 646 nm)
Water Content (KF)	≤5.0%
Solubility	Freely soluble in water, PBS, and other aqueous buffers
Extinction Coefficient (ε)	≥250,000 M-1cm-1 at λmax in water
Fluorescence Emission	~662 nm (in aqueous buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.