Description

n-(Azido-Peg3)-n-Boc-Peg4-Nhs Ester CAS NO 2112731-96-9 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester, separated by a Boc-protected amine on a tetraethylene glycol spacer. This compound is critical for creating stable, site-specific bioconjugates through copper-free click chemistry and amine-reactive coupling. It is primarily needed by researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science for labeling, surface functionalization, and constructing drug delivery systems.

Application

• Bioconjugation and Protein Labeling: Site-specific attachment of fluorescent dyes, peptides, or drugs to antibodies, proteins, and other biomolecules via the NHS ester and subsequent click chemistry with alkynes.
• ADC (Antibody-Drug Conjugate) Linker Development: Serves as a cleavable or non-cleavable PEG-based linker to connect cytotoxic payloads to monoclonal antibodies, improving solubility and pharmacokinetics.
• Surface Modification and Biointerface Engineering: Functionalization of nanoparticles, biosensors, and microarray slides to create bioactive surfaces for diagnostic and research applications.
• Polymer and Hydrogel Synthesis: Building block for synthesizing functionalized PEG-based polymers and crosslinked hydrogels with tailored biological properties.
• Chemical Biology Probes: Construction of activity-based probes for studying enzyme function and protein-protein interactions in live cells.
• Diagnostic Reagent Formulation: Development of sensitive immunoassay components and imaging agents.

Basic Information

Product Name	n-(Azido-Peg3)-n-Boc-Peg4-Nhs Ester
CAS No.	2112731-96-9
Molecular Formula	C31H55N5O13
Molecular Weight	705.80 g/mol
Synonyms	1-Azido-3,6,9,12,15,18,21,24-octaoxaheptacosan-27-yl (2,5-dioxopyrrolidin-1-yl) (tert-butoxycarbonyl)carbamate; Azido-PEG3-Boc-PEG4-NHS ester; Boc-N-amido-PEG4-NHS ester-PEG3-azide; N-(Azido-PEG3)-N-Boc-PEG4-NHS ester; Heterobifunctional PEG crosslinker, Azide-Boc-NHS; 2,5-Dioxopyrrolidin-1-yl 1-[(1,1-dimethylethoxy)carbonyl]-3,6,9,12,15,18,21,24-octaoxa-27-azaoctacosan-27-oate; Boc-NH-PEG4-NHS-PEG3-N3
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Boc-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for research and bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.