Description

n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5 CAS NO 2107273-58-3 is a sophisticated, multi-functional heterobifunctional crosslinker designed for advanced bioconjugation and labeling applications. Its core value lies in the precise integration of a cyanine 5 (Cy5) fluorophore with multiple polyethylene glycol (PEG) spacers and a terminal azide group, enabling controlled, site-specific labeling via click chemistry. This compound is essential for researchers and developers in the fields of biopharmaceuticals, diagnostics, and advanced materials science who require high-purity, functional linkers for creating stable, fluorescently tagged biomolecules.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Site-specific labeling of antibodies, proteins, and peptides for therapeutic and diagnostic applications.
• Fluorescent Probe and Biosensor Fabrication: Creating highly sensitive detection probes for in vitro and in vivo imaging, including fluorescence microscopy and flow cytometry.
• Biomaterial and Hydrogel Functionalization: Incorporating fluorescent tags into polymers and scaffolds for tracking and visualization in tissue engineering.
• Click Chemistry-Based Labeling: Utilizing the terminal azide group for copper-catalyzed or strain-promoted azide-alkyne cycloaddition (SPAAC) with alkyne-modified molecules.
• Nucleic Acid Labeling: Modification of oligonucleotides for fluorescence in situ hybridization (FISH) and other genomic analysis techniques.
• Drug Delivery System Tracking: Labeling liposomes, nanoparticles, or other delivery vehicles to monitor biodistribution and pharmacokinetics.
• Protein-Protein Interaction Studies: Employing as a crosslinking agent for analyzing molecular interactions in complex biological systems.

Basic Information

Item	Details
Product Name	n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5
CAS No.	2107273-58-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5-PEG4-Azide; Azide-PEG4-Cy5; Cyanine5 azide PEG4 crosslinker; m-PEG4-Cy5-N3; Heterobifunctional Cy5 PEG azide; 2107273-58-3; Azido-PEG4-Cy5 dye; Cy5-PEG4-N3 linker
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and mass spectrometry for structural confirmation, and functional group analysis. Certificates of Analysis (COA) detailing purity, identity, and performance characteristics are available upon request to ensure compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 M⁻¹cm⁻¹ (at λmax ~649 nm)
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water
Functional Group Assay (Azide)	≥ 90%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.