Description

n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5 is a bifunctional, near-infrared fluorescent labeling reagent engineered for advanced bioconjugation. This compound matters for researchers and developers requiring precise, stable, and efficient labeling of biomolecules for sensitive detection and imaging applications. It is primarily needed in the pharmaceutical, biotechnology, and diagnostic industries for applications such as antibody-drug conjugate (ADC) development, protein/peptide labeling, and in vivo imaging studies. CAS No. 2107273-56-1.

Application

• Antibody-Drug Conjugate (ADC) Development: Used as a fluorescent linker-payload for tracking conjugation efficiency, stability, and cellular uptake of ADCs.
• Protein and Peptide Labeling: Enables site-specific fluorescent tagging of proteins and peptides via copper-free click chemistry (azide-alkyne cycloaddition) for structural and functional studies.
• In Vivo and In Vitro Imaging: Serves as a near-infrared (NIR) probe for deep-tissue fluorescence imaging, flow cytometry, and microscopy due to the Cy5 fluorophore's properties.
• Diagnostic Assay Development: Utilized in the creation of fluorescent immunoassays, biosensors, and other diagnostic tools requiring high-sensitivity detection.
• Polymer and Material Science: Acts as a functional modifier for introducing fluorescence and click-chemistry handles onto surfaces, nanoparticles, or hydrogels.
• Chemical Biology Probes: Facilitates the study of biomolecular interactions, localization, and dynamics in complex biological systems.

Basic Information

Product Name	n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5
CAS No.	2107273-56-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5-PEG4-azide PEG4 methyl linker; NIR fluorescent PEG azide; Azido-PEG4-Cy5 methyl derivative; m-PEG4-Cy5-azide; Bifunctional Cy5 labeling reagent; Cy5 azide PEG4 methyl ether; Fluorescent click chemistry reagent 2107273-56-1; Methyl-PEG4-Cy5-N3
EINECS	Contact for details

Quality Control

Our n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy5 is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity for research and development. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and spectroscopic methods (NMR, MS) for structural confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.