Description

n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy5 is a sophisticated, multi-functional cyanine dye derivative engineered for advanced bioconjugation and labeling applications. This compound matters because it integrates a Cy5 fluorophore with multiple polyethylene glycol (PEG) spacers and a propargyl group, offering superior water solubility, reduced non-specific binding, and a specific click chemistry handle for site-specific labeling. It is essential for researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and advanced materials science who require high-performance, customizable fluorescent probes.

Application

• Bioconjugation and Probe Development: Site-specific labeling of antibodies, proteins, peptides, and oligonucleotides via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• In Vivo and In Vitro Imaging: Fluorescent tagging for near-infrared (NIR) fluorescence imaging, flow cytometry, and microscopy due to the Cy5 fluorophore's excellent photostability and emission profile.
• Drug Delivery System Tracing: Conjugation to nanoparticles, liposomes, or polymer-drug conjugates to track biodistribution, pharmacokinetics, and cellular uptake.
• Diagnostic Assay Reagents: Key component in developing high-sensitivity lateral flow assays (LFAs), ELISA, and other immunoassays requiring stable, bright fluorescent signals.
• Biomaterial and Hydrogel Functionalization: Incorporation into smart materials and scaffolds for biomedical research to visualize material integration and cell interactions.
• Chemical Biology and Proteomics: Tool for activity-based protein profiling (ABPP) and studying biomolecular interactions in complex biological systems.

Basic Information

Item	Details
Product Name	n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy5
CAS No.	2107273-54-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5-PEG4-Propargyl; Propargyl-PEG4-Cy5; Cyanine5 alkyne PEG derivative; NIR fluorescent alkyne tag; m-PEG4-Cy5-Propargyl; Cy5-PEG4-alkyne probe; Functionalized Cy5 dye for click chemistry
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(m-Peg4)-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy5 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are available upon request. We adhere to cGMP principles where applicable to support our customers in regulated research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Area % at specified wavelength)
Water Content (KF)	≤ 1.0 %
Functional Group Integrity	Confirmed by NMR or other appropriate method

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.