Description

N,N'-Bis-(Azide-Peg3)-Cy5 is a bifunctional, near-infrared fluorescent labeling reagent designed for bioorthogonal conjugation via click chemistry. This compound matters for researchers and developers requiring precise, stable, and highly detectable linkages in complex biological systems. It is primarily needed by professionals in bioconjugation, pharmaceutical R&D, and diagnostic assay development for creating advanced probes and targeted therapeutics.

Application

• Bioconjugation and Probe Development: Used for site-specific labeling of antibodies, proteins, peptides, and oligonucleotides with a near-infrared fluorescent Cy5 dye.
• Click Chemistry Applications: The dual azide (N3) groups enable efficient copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) with alkyne-modified biomolecules or surfaces.
• In Vivo and In Vitro Imaging: Serves as a critical component in synthesizing NIR fluorescent probes for deep-tissue imaging, cell tracking, and molecular diagnostics.
• Pharmaceutical Research: Facilitates the development of antibody-drug conjugates (ADCs) and other targeted drug delivery systems by providing a fluorescent linker for pharmacokinetic studies.
• Diagnostic Assay Manufacturing: Employed in the production of fluorescent lateral flow assays, microarrays, and other high-sensitivity diagnostic tools.
• Materials Science: Used for functionalizing nanoparticles, polymers, and hydrogels with fluorescent properties for biosensing and biointerface studies.

Basic Information

Product Name	N,N'-Bis-(Azide-Peg3)-Cy5
CAS No.	2107273-46-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5-azide-PEG3-azide; Bis-Azide-PEG3-Cy5; N,N'-Bis(azido-PEG3)-Cyanine5; Azide-PEG3-Cy5-PEG3-Azide; Cy5 bis-PEG3 azide; 2107273-46-9; CyaninedyeazidePEG3azide
EINECS	Contact for details

Quality Control

Every batch of N,N'-Bis-(Azide-Peg3)-Cy5 undergoes rigorous analytical testing to ensure high purity and consistent performance for critical research applications. Our quality assurance includes HPLC purity analysis, mass spectrometry (MS) confirmation, and functional validation of the azide groups. Certificates of Analysis (COA) detailing batch-specific results are available upon request, supporting compliance with internal quality standards for bioconjugation reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is light-sensitive and hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Moisture Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	Conforms
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.