Description

Bis-(N,N'-Carboxyl-Peg4)-Cy5 CAS NO 2107273-44-7 is a bifunctional, near-infrared fluorescent labeling reagent featuring two carboxylic acid groups linked via tetraethylene glycol (PEG4) spacers to a Cy5 cyanine dye core. This compound is engineered to provide superior water solubility and reduced non-specific binding, making it an essential tool for creating stable, high-performance bioconjugates. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, in vivo imaging, and advanced diagnostic assay production.

Application

• Bioconjugation and Probe Synthesis: Used for labeling antibodies, proteins, peptides, and oligonucleotides with a near-infrared fluorescent tag for detection and imaging.
• In Vivo and In Vitro Imaging: Enables deep-tissue fluorescence imaging in preclinical research due to the Cy5 dye's optimal excitation/emission profile.
• Diagnostic Assay Development: Serves as a critical component in developing fluorescence-based immunoassays, flow cytometry kits, and microarray platforms.
• Drug Delivery System Tracking: Facilitates the study of pharmacokinetics and biodistribution of nanoparticle or polymer-based drug carriers.
• Biomolecular Interaction Studies: Applied in fluorescence polarization (FP) and Förster resonance energy transfer (FRET) assays to study molecular interactions.
• Cell Labeling and Tracking: Used for long-term, non-cytotoxic labeling of cells for migration and proliferation studies.

Basic Information

Product Name	Bis-(N,N'-Carboxyl-Peg4)-Cy5
CAS No.	2107273-44-7
Molecular Formula	C58H78N4O16
Molecular Weight	1063.27 g/mol
Synonyms	Bis-Carboxy-PEG4-Cy5; Cy5-PEG4-diacid; Cyanine5 bis(PEG4-acid); 1,1'-(2,5,8,11,14,17,20,23-Octaoxapentacosane-1,25-diyl)bis(1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-5-((E)-1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium); Bis(NHS-PEG4)-Cy5 precursor; Cy5 dicarboxylic acid PEG derivative; Fluorescent PEG linker Cy5.
EINECS	Contact for details

Quality Control

Our Bis-(N,N'-Carboxyl-Peg4)-Cy5 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional suitability. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements of research and diagnostic-grade chemical synthesis.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to prevent condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient	≥ 250,000 cm-1M-1 at ~649 nm
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMSO, DMF, water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.