Description

n-(m-Peg4)-N'-(Peg2-Acid)-Cy5 CAS NO 2107273-24-3 is a heterobifunctional polyethylene glycol (PEG) linker featuring a maleimide (m) group for thiol conjugation and a carboxylic acid (Peg2-Acid) for amine coupling, with a deep-red cyanine 5 (Cy5) fluorophore for detection. This compound is engineered to enable the controlled, site-specific labeling of biomolecules such as proteins and antibodies, facilitating advanced bioconjugation strategies. It is essential for researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and life science research who require high-performance fluorescent probes for imaging, tracking, and analytical applications.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or fluorescent tags to monoclonal antibodies for targeted cancer therapies.
• Protein Labeling & Bioconjugation: Covalent attachment of the Cy5 fluorophore to proteins, peptides, or enzymes for fluorescence microscopy, flow cytometry, and in vivo imaging.
• Diagnostic Probe Synthesis: Creation of fluorescent probes and biosensors for immunoassays (e.g., ELISA), lateral flow tests, and molecular diagnostics.
• Biomaterial & Nanoparticle Functionalization: Surface modification of nanoparticles, liposomes, or polymeric carriers to create targeted delivery systems with traceable fluorescent properties.
• Biophysical Studies: Fluorescent labeling of biomolecules to study protein-protein interactions, binding kinetics, and cellular uptake mechanisms.
• Next-Generation Sequencing (NGS): Potential use in the preparation of fluorescently labeled nucleotides or probes for genomic analysis.

Basic Information

Item	Details
Product Name	n-(m-Peg4)-N'-(Peg2-Acid)-Cy5
CAS No.	2107273-24-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Maleimide-PEG4-PEG2-Acid-Cy5; Cy5-PEG4-Maleimide-PEG2-COOH; mPEG4-PEG2-Acid-Cyanine5; Heterobifunctional PEG-Cy5 linker, Maleimide-PEG6-Acid-Cy5 (common alias); 1-(3-{2-[2-(3-{2-[2-(2-{5-[(1E,3E,5E)-5-(1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethyl-5-sulfo-1,3-dihydro-2H-indol-2-ylidene)penta-1,3-dien-1-yl]-1,3,3-trimethyl-3H-indol-1-ium-2-yl}ethoxy)ethoxy]ethoxy}propoxy)ethoxy]ethoxy}propyl)-1H-pyrrole-2,5-dione (IUPAC-style); Cy5 NHS ester-PEG-maleimide derivative; Fluorescent PEG crosslinker
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Peg2-Acid)-Cy5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility for critical research and development applications. Certificates of Analysis (COA) providing detailed results for purity (HPLC), identity (MS, NMR), and functional integrity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 cm⁻¹M⁻¹ (at λmax ~649 nm)
Functional Group Integrity	Maleimide > 90%, Acid > 90%
Solubility	Soluble in DMSO, DMF, acetonitrile, water (variable)
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.