Description

n-(m-Peg4)-N'-(Hydroxy-Peg2)-Cy5 is a bifunctional, water-soluble cyanine dye derivative engineered for advanced bioconjugation and labeling applications. This compound matters for its dual functionality, combining a maleimide group for thiol-specific coupling and a hydroxyl group for further chemical modification, offering researchers precise control over biomolecule tagging. It is primarily needed by professionals in biopharmaceutical R&D, diagnostic assay development, and advanced life sciences for creating stable, fluorescent probes and conjugates.

Application

• Fluorescent Labeling of Proteins and Antibodies: Site-specific conjugation via the maleimide group to cysteine residues for flow cytometry, immunofluorescence, and Western blotting.
• Development of Diagnostic Assays: Creation of fluorescent probes and sensors for ELISA, lateral flow assays, and in vitro diagnostics (IVD).
• Live-Cell and In Vivo Imaging: Synthesis of near-infrared (NIR) imaging agents due to the Cy5 fluorophore's deep tissue penetration and low background.
• Polymer and Nanoparticle Functionalization: Modification of PEGylated surfaces, drug delivery carriers, and polymeric materials for traceable biodistribution studies.
• Nucleic Acid Labeling: Preparation of fluorescently tagged oligonucleotides and probes for FISH (Fluorescence In Situ Hybridization) and real-time PCR.
• Bioconjugation Tool for Chemical Biology: A versatile linker in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic constructs.

Basic Information

Item	Detail
Product Name	n-(m-Peg4)-N'-(Hydroxy-Peg2)-Cy5
CAS No.	2107273-12-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5 maleimide-PEG4-hydroxy-PEG2; Maleimide-PEG4-hydroxy-PEG2-Cyanine5; Hydroxy-PEG2-Cy5-maleimide-PEG4; Cy5-PEG4-MAL-PEG2-OH; N-(Maleimide-PEG4)-N'-(Hydroxy-PEG2)-Cy5; Bifunctional Cy5 PEG linker; Cy5 dye heterobifunctional crosslinker; Succinimidyl-PEG4-Cy5-PEG2-hydroxy (common naming variant)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and diagnostic use. Each batch is characterized by advanced analytical techniques including HPLC and mass spectrometry to verify identity, purity, and functional integrity. Certificates of Analysis (COA) detailing purity, absorbance, and functional group activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 cm⁻¹M⁻¹ (typical for Cy5 dyes)
Functional Group Integrity	Maleimide > 90% active
Water Content (KF)	< 1.0%
Solubility	Soluble in DMSO, DMF, water, and common polar organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.