Description

n-(m-Peg9)-N'-(Propargyl-Peg8)-Cy5 is a heterobifunctional, near-infrared fluorescent labeling reagent designed for advanced bioconjugation and biomolecular assembly. This compound matters for researchers and developers requiring precise, site-specific attachment of fluorophores or other functional groups to biomolecules via click chemistry and amine-reactive chemistry. It is primarily needed by professionals in biopharmaceutical R&D, diagnostic assay development, and advanced materials science for applications such as protein/antibody labeling, nucleic acid probes, and the construction of complex biomolecular architectures.

Application

• Bioconjugation & Protein Labeling: Site-specific fluorescent labeling of antibodies, proteins, and peptides for imaging, flow cytometry, and immunoassays.
• Click Chemistry Probes: Utilizes the propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) to attach Cy5 fluorophores to azide-modified molecules.
• Nucleic Acid Labeling: Modification of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Polymer & Dendrimer Functionalization: Building block for synthesizing fluorescently tagged PEGylated polymers and dendrimers for drug delivery systems.
• Live-Cell & In Vivo Imaging: Creation of NIR probes for deep-tissue imaging due to the Cy5 fluorophore's favorable spectral properties.
• Diagnostic Kit Development: Key reagent in the manufacture of fluorescent lateral flow assays and other point-of-care diagnostic devices.
• Surface Modification: Grafting fluorescent PEG layers onto biosensor surfaces or nanoparticles for enhanced biocompatibility and tracking.

Basic Information

Product Name	n-(m-Peg9)-N'-(Propargyl-Peg8)-Cy5
CAS No.	2107273-10-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5-PEG9-NHS ester-PEG8-Alkyne; Maleimide-PEG9-Cy5-PEG8-Propargyl; NHS-PEG9-Cy5-PEG8-Alkyne; Propargyl-PEG8-Cy5-PEG9-NHS ester; Heterobifunctional Cy5-PEG-Alkyne/NHS reagent; Cy5-PEG9-NHS-PEG8-Propargyl; N-(mPEG9)-N'-(Propargyl-PEG8)-Cyanine5; Amine & Alkyne reactive Cy5 PEG crosslinker
EINECS	Contact for details

Quality Control

Our n-(m-Peg9)-N'-(Propargyl-Peg8)-Cy5 is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional group analysis. Certificates of Analysis (COA) detailing purity, substitution ratios, and spectral data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 M⁻¹cm⁻¹ (at λmax ~649 nm)
Functional Group Substitution	NHS ester & Propargyl content confirmed
Solubility	Soluble in DMSO, DMF, acetonitrile, water (with sonication)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.