Description

n-Methyl-N'-(Propargyl-Peg4)-Cy5 is a specialized, high-purity fluorescent labeling reagent designed for advanced bioconjugation and bioimaging applications. This compound matters because it combines a reactive propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry with a hydrophilic polyethylene glycol (PEG4) spacer and a far-red emitting cyanine (Cy5) fluorophore, enabling precise, efficient, and biocompatible tagging of biomolecules. It is essential for researchers and developers in pharmaceutical R&D, diagnostic assay development, and life science research who require reliable, site-specific labeling for probes, antibodies, and nanoparticles.

Application

• Fluorescent Labeling of Biomolecules: Site-specific conjugation to antibodies, proteins, peptides, and oligonucleotides via click chemistry for imaging and detection.
• Live-Cell and In Vivo Imaging Probes: Development of targeted imaging agents utilizing the far-red emission of Cy5 for deep tissue penetration and minimal background autofluorescence.
• Diagnostic Assay Development: Creation of fluorescent conjugates for ELISA, flow cytometry, immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH).
• Polymer and Nanoparticle Functionalization: Modification of drug delivery carriers, dendrimers, and polymeric materials with traceable fluorescent tags.
• Proteomics and Biomarker Discovery: Labeling substrates for activity-based protein profiling (ABPP) and other chemical proteomics strategies.
• Bioconjugation Tool for ADC Development: As a model payload-linker component in the research and optimization of antibody-drug conjugates (ADCs).

Basic Information

Product Name	n-Methyl-N'-(Propargyl-Peg4)-Cy5
CAS No.	2107273-04-9
Molecular Formula	C52H68N4O9
Molecular Weight	893.1 g/mol
Synonyms	Propargyl-PEG4-Cy5 alkyne; Cy5 alkyne, PEG4; N-Methyl-N'-(propargyl-PEG4)-Cy5; Sulfo-Cyanine5 alkyne, PEG4; 1-(Prop-2-yn-1-yl)-3,6,9,12-tetraoxapentadec-14-yl (E)-2-((E)-2-(2-((E)-2-(1-methyl-1H-indol-3-yl)vinyl)-4-((E)-2-(1-methyl-1,2-dihydroquinolin-6-yl)vinyl)quinolin-1-ium-1-yl)ethyl)-4-methylpiperazine-1-carboxylate; Cy5-PEG4-Alkyne; Alkyne-PEG4-Cy5
EINECS	Contact for details

Quality Control

Our n-Methyl-N'-(Propargyl-Peg4)-Cy5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is strictly light-sensitive and hygroscopic. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.