Description

2,5-Dioxopyrrolidin-1-Yl 3-Methyl-3-(Pyridin-2-Yldisulfanyl)Butanoate is a specialized heterobifunctional crosslinking reagent featuring a reactive NHS ester and a cleavable pyridyl disulfide group. This molecular design is critical for creating stable, reversible conjugates between amine-containing molecules and thiol groups. It is an essential tool for researchers and manufacturers in bioconjugation, enabling the development of advanced drug delivery systems, diagnostic assays, and functionalized biomaterials.

Application

• Bioconjugation and Protein Modification: Primary application for site-specific crosslinking of antibodies, enzymes, and other proteins to drugs, polymers, or surfaces via amine-to-thiol coupling.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key intermediate for constructing cleavable linkers that connect cytotoxic payloads to monoclonal antibodies, leveraging the reducible disulfide bond for targeted drug release.
• Surface Functionalization: Used to immobilize biomolecules onto biosensor chips, nanoparticles, or chromatography resins by creating a reversible covalent attachment.
• Polymer and Dendrimer Synthesis: Enables the controlled assembly and functionalization of macromolecular structures for drug delivery and material science applications.
• Diagnostic Reagent Development: Critical for labeling peptides, proteins, and oligonucleotides with fluorophores or other reporter molecules in assay development.
• Prodrug Synthesis: Facilitates the creation of prodrugs where an active pharmaceutical ingredient is reversibly masked via a disulfide linkage for controlled activation.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 3-Methyl-3-(Pyridin-2-Yldisulfanyl)Butanoate
CAS No.	2101206-29-3
Molecular Formula	C14H16N2O4S2
Molecular Weight	340.42 g/mol
Synonyms	NHS-Pyridyl Disulfide; 3-Methyl-3-(2-pyridyldithio)butyric acid N-hydroxysuccinimide ester; SPP-NHS; N-Succinimidyl 3-(2-pyridyldithio)propionate analog; Heterobifunctional NHS-Pyridyldithiol crosslinker; Pyridyl disulfide NHS ester; 3-Methyl-3-(pyridin-2-yldisulfanyl)butanoic acid 2,5-dioxopyrrolidin-1-yl ester; N-(3-(2-Pyridyldithio)propionyloxy)succinimide derivative
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized using advanced analytical techniques to guarantee identity, purity, and functional performance. Certificates of Analysis (COA) detailing specific results for purity (HPLC), identity (NMR, IR), and other critical parameters are available upon request. We adhere to cGMP guidelines for the production of materials intended for pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.