Description

Nhpi-Peg3-C2-Nhs Ester is a heterobifunctional crosslinker featuring an amine-reactive NHS ester and a click chemistry-compatible terminal alkyne group, connected by a hydrophilic triethylene glycol (PEG3) spacer. This molecular architecture is critical for creating stable, site-specific conjugates in aqueous environments while minimizing non-specific binding. It is an essential reagent for researchers and manufacturers in bioconjugation, proteomics, and ADC (Antibody-Drug Conjugate) development who require precise and efficient coupling strategies.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a key building block for constructing cleavable or non-cleavable linkers, connecting cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Enables the site-specific modification of lysine residues on proteins or peptides with fluorescent dyes, biotin, or other functional probes via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Surface Functionalization: Used to immobilize biomolecules onto azide-modified surfaces (e.g., biosensors, microarrays, nanoparticles) for creating bioactive interfaces.
• Polymer Conjugation: Facilitates the grafting of functional polymers onto amine-containing biomolecules or materials to enhance solubility, stability, or stealth properties.
• Diagnostic Reagent Synthesis: Employed in the development of diagnostic assays and imaging probes that require stable, well-defined chemical linkages.
• Chemical Biology Tool Development: A versatile reagent for synthesizing activity-based probes or affinity tags to study protein function and interactions.

Basic Information

Product Name	Nhpi-Peg3-C2-Nhs Ester
CAS No.	2101206-14-6
Molecular Formula	C₂₆H₃₃N₃O₉
Molecular Weight	531.56 g/mol
Synonyms	2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(3-(2-(prop-2-yn-1-yloxy)propanamido)propanamido)ethoxy)ethoxy)propanoate; N-Hydroxysuccinimide ester of PEG3 alkyne; NHS-PEG3-Alkyne; Alkyne-PEG3-NHS ester; 1-(3-(2-(2-(3-(2-(Prop-2-yn-1-yloxy)propanamido)propanamido)ethoxy)ethoxy)propanoyl)-2,5-pyrrolidinedione; Propargyl-PEG3-NHS; N-(Prop-2-yn-1-yl)-PEG3-NHS ester
EINECS	Contact for details

Quality Control

Every batch of Nhpi-Peg3-C2-Nhs Ester is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Our rigorous quality testing ensures compliance with specifications critical for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.