Description

Dbco-Peg4-Nhs Ester is a heterobifunctional crosslinking reagent featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and an NHS ester for efficient amine coupling. This compound is essential for creating stable, bioconjugate linkages without the need for cytotoxic copper catalysts, enabling the labeling of sensitive biomolecules. It is primarily needed by researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic probe synthesis.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies.
• Fluorescent Probe Labeling: For attaching fluorophores to proteins, peptides, or oligonucleotides via bioorthogonal chemistry.
• Surface Functionalization: For modifying nanoparticles, biosensors, or microarray slides with bioactive ligands.
• Polymer and Dendrimer Modification: For introducing functional groups into macromolecular structures for drug delivery systems.
• Peptide and Protein PEGylation: For installing polyethylene glycol (PEG) chains to improve pharmacokinetic properties.
• Diagnostic Reagent Synthesis: For developing immunoassay components and molecular imaging agents.

Basic Information

Product Name	Dbco-Peg4-Nhs Ester
CAS No.	2100306-58-7
Molecular Formula	C₃₃H₄₀N₂O₁₀
Molecular Weight	624.68 g/mol
Synonyms	DBCO-PEG4-NHS Ester; DBCO-PEG4-NHS; Dibenzocyclooctyne-PEG4-N-hydroxysuccinimide ester; 1-(11,12-Didehydrodibenzo[b,f]azocin-5(6H)-yl)-3,6,9,12-tetraoxapentadecan-15-oic acid 2,5-dioxopyrrolidin-1-yl ester; DBCO-PEG₄-NHS; Azadibenzocyclooctyne-PEG4-NHS; ADIBO-PEG4-NHS Ester; DBCO-PEG4-SE
EINECS	Contact for details

Quality Control

Our Dbco-Peg4-Nhs Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for critical research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management standards, and our processes are designed to support cGMP manufacturing for pre-clinical and clinical-stage bioconjugate projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.