Description

n-(Azido-Peg4)-n-Bis(Peg4-T-Butyl Ester) is a specialized heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and two protected carboxylic acid termini. This compound is engineered for high-efficiency, site-specific bioconjugation, particularly in click chemistry applications where its water-solubility and biocompatibility are critical. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science who require precise and stable linkages for creating antibody-drug conjugates (ADCs), peptide modifications, and hydrogel networks.

Application

• Bioconjugation and ADC Development: Site-specific coupling of payloads to antibodies or proteins via strain-promoted azide-alkyne cycloaddition (SPAAC) or copper-catalyzed click chemistry (CuAAC).
• PEGylation Reagent: Modification of peptides, proteins, and small molecules to enhance solubility, stability, and circulation half-life.
• Crosslinking Agent for Hydrogels: Formation of biocompatible, tunable hydrogel matrices for 3D cell culture, drug delivery systems, and tissue engineering scaffolds.
• Surface Functionalization: Immobilization of biomolecules onto nanoparticles, biosensors, and diagnostic assay platforms.
• Polymer Chemistry: Building block for synthesizing functionalized, multi-arm PEG polymers with controlled architectures.
• Chemical Biology Probes: Synthesis of activity-based probes for labeling and studying biological targets in live cells.

Basic Information

Product Name	n-(Azido-Peg4)-n-Bis(Peg4-T-Butyl Ester)
CAS No.	2093152-79-3
Molecular Formula	C41H77N5O18
Molecular Weight	928.07 g/mol
Synonyms	Azido-PEG4-tris-(t-butyl-PEG4-ester); 1-Azido-3,6,9,12-tetraoxapentadecane-15,15-bis(2,5,8,11,14-pentaoxahexadecan-16-oic acid t-butyl ester); Heterobifunctional PEG Crosslinker, Azide-(PEG4)-tris(t-butyl ester-PEG4); N3-PEG4-tris(PEG4-OtBu); Azido-PEG4-tris(carboxy-PEG4-t-butyl ester); PEG-based Azide and Protected Acid Crosslinker
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg4)-n-Bis(Peg4-T-Butyl Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for critical research and development applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and hydrolysis of the t-butyl ester protecting groups. Allow the product to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.