Description

Alexa Fluor 647 Acid is a high-performance, far-red fluorescent dye derivative, widely recognized for its exceptional brightness and photostability. This carboxylic acid-functionalized probe is a critical reagent for creating stable, site-specific conjugates with biomolecules, enabling superior signal-to-noise ratios in demanding assays. It is essential for researchers and manufacturers in the fields of flow cytometry, super-resolution microscopy, and in vivo imaging who require reliable, high-quality labeling reagents.

Application

• Antibody and Protein Labeling: Conjugation to primary or secondary antibodies for multiplex flow cytometry and immunofluorescence microscopy.
• Nucleic Acid Probes: Labeling of oligonucleotides (FISH probes, primers) for genetic analysis and diagnostics.
• Cell Tracking and Imaging: Long-term cell tracing and in vivo imaging applications due to its deep tissue penetration and stability.
• Microarray and Biochip Fabrication: Immobilization of biomolecules on sensor surfaces for high-throughput screening.
• Super-Resolution Microscopy (STORM, PALM): Provides the photophysical properties required for single-molecule localization techniques.
• Diagnostic Assay Development: Key component in the development of clinical and research diagnostic kits requiring far-red detection channels.

Basic Information

Product Name	Alexa Fluor 647 Acid
CAS No.	2090339-69-6
Molecular Formula	C37H44KN4O14S4
Molecular Weight	~ 1000.2 g/mol (as potassium salt)
Synonyms	Alexa Fluor® 647 Carboxylic Acid; Alexa Fluor 647 SE precursor; AF647 Acid; 6-((6-((3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)(ethyl)amino)-2,3-dihydro-1H-xanthen-4-yl)(ethyl)amino)-6-oxohexanoic acid (precursor form); Succinimidyl ester of Alexa Fluor 647 carboxylic acid (related compound); Far-red fluorescent dye acid; Life Technologies A-20006 (related catalog number)
EINECS	Contact for details

Quality Control

Every batch of Alexa Fluor 647 Acid is manufactured and tested under stringent quality management systems to ensure high purity, consistent performance, and batch-to-batch reproducibility. Our quality control includes rigorous analysis by HPLC to confirm identity, purity, and the absence of unreacted precursors or isomers. Certificates of Analysis (COA) detailing spectral properties (Absorption/Emission Maxima, Extinction Coefficient) and functional performance data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	650 ± 3 nm
Emission Maximum (λem)	668 ± 3 nm
Extinction Coefficient (ε)	~270,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0%
Functional Performance (Labeling)	Conjugates efficiently with antibodies/amines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.