Description

n-(Azido-Peg2)-n-Fluorescein-Peg4-Acid CAS NO 2086689-06-5 is a bifunctional, heterobifunctional crosslinker that combines a reactive azide group with a fluorescent fluorescein reporter and a terminal carboxylic acid. This compound is engineered for high-efficiency bioconjugation, enabling researchers to label biomolecules with a bright, photostable fluorophore while introducing a click chemistry handle for further modification. It is essential for scientists in bioconjugation, proteomics, and advanced materials science who require precise, traceable labeling and crosslinking strategies.

Application

• Bioconjugation and Protein Labeling: Covalent attachment of fluorescent tags to proteins, antibodies, or peptides via the carboxylic acid group for subsequent click chemistry with alkyne-modified surfaces or molecules.
• Click Chemistry Probes: Serving as a key reagent in copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions for bioorthogonal labeling.
• Fluorescent Sensor Development: Integration into molecular scaffolds for creating sensors that detect enzymatic activity, pH changes, or specific analytes.
• Cell Imaging and Tracking: Labeling of cell surface proteins or internal structures for visualization and tracking in live-cell or fixed-cell microscopy (e.g., confocal, fluorescence).
• Polymer and Materials Functionalization: Modification of nanoparticles, hydrogels, or polymer backbones to introduce fluorescence and reactive sites for further chemical grafting.
• Diagnostic Assay Development: Used in the fabrication of fluorescent immunoassays, lateral flow tests, or other diagnostic platforms requiring stable, bright signal generation.
• Drug Delivery System Research: Conjugation to drug carriers or targeting ligands to create traceable, multifunctional delivery systems.

Basic Information

Product Name	n-(Azido-Peg2)-n-Fluorescein-Peg4-Acid
CAS No.	2086689-06-5
Molecular Formula	C52H61N5O18
Molecular Weight	1044.1 g/mol
Synonyms	5-FAM-PEG4-Azide; 5-Carboxyfluorescein-PEG4-Azide; Azido-PEG2-Fluorescein-PEG4-Acid; Fluorescein-PEG4-N3; 6-Isomer of FAM-PEG4-Azide; Azido-PEG2-C2-Amine-Fluorescein-PEG4-Acid; N-(Azido-PEG2)-N'-Fluorescein-PEG4-Acid; Heterobifunctional PEG Crosslinker, Azide-Fluorescein-Acid
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg2)-n-Fluorescein-Peg4-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.