Description

Tco-Peg24-Nhs Ester is a heterobifunctional crosslinking reagent featuring a trans-cyclooctene (TCO) group for bioorthogonal click chemistry and an NHS ester for efficient amine conjugation. This compound is essential for creating stable, site-specific bioconjugates under mild, aqueous conditions without the need for cytotoxic catalysts. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science for labeling proteins, antibodies, and nanoparticles.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins for therapeutic and diagnostic applications.
• Pretargeted Imaging and Therapy: A key component in pretargeting strategies for in vivo imaging (e.g., PET, SPECT) and radioimmunotherapy due to its fast and specific reaction with tetrazines.
• Surface Functionalization: Immobilization of biomolecules onto chips, beads, or nanoparticles for biosensor and microarray development.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Used in research for developing novel cleavable or non-cleavable linker systems in ADC platforms.
• Polymer and Biomaterial Modification: Introduction of bioorthogonal handles into PEG-based hydrogels and other polymeric materials for subsequent cell or drug attachment.
• Chemical Biology Probes: Synthesis of activity-based probes and other tools for studying biological processes and target engagement.

Basic Information

Product Name	Tco-Peg24-Nhs Ester
CAS No.	2055646-26-7
Molecular Formula	C58H100N2O28
Molecular Weight	1249.4 g/mol
Synonyms	NHS-PEG24-TCO; TCO-PEG24-NHS Ester; trans-Cyclooctene-PEG24-N-hydroxysuccinimide ester; (E)-Cyclooct-4-enyl-PEG24-NHS; 2,5-Dioxopyrrolidin-1-yl 3-((2-(2-(2-((1-((E)-cyclooct-4-en-1-yl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethoxy)ethyl)thio)propanoate; TCO-PEG24-OSu; Heterobifunctional PEG linker TCO-PEG-NHS
EINECS	Contact for details

Quality Control

Our Tco-Peg24-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure consistency and performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (NHS Ester)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.