Description

Trisulfo-Cy5-Alkyne is a near-infrared fluorescent dye derivative functionalized with an alkyne group for bioorthogonal click chemistry. This compound is engineered for high aqueous solubility and photostability, making it a critical tool for advanced bioconjugation and labeling applications. It is essential for researchers and developers in the fields of bioimaging, diagnostics, and pharmaceutical development who require sensitive and specific probes for tracking biomolecules in complex biological systems.

Application

• Bioorthogonal Labeling: Conjugation to azide-tagged biomolecules (proteins, antibodies, nucleic acids) via copper-catalyzed or copper-free click chemistry for fluorescence tagging.
• Live-Cell and In Vivo Imaging: Utilization as a near-infrared (NIR) probe for deep-tissue imaging with minimal background autofluorescence.
• Diagnostic Assay Development: Integration into fluorescence-based immunoassays, flow cytometry, and microscopy for high-sensitivity detection.
• Proteomics and Biomarker Discovery: Labeling of newly synthesized proteins or other cellular components for tracking and analysis.
• Pharmaceutical Research: Conjugation to drug candidates or nanoparticles for studying biodistribution, pharmacokinetics, and target engagement.
• Material Science: Functionalization of surfaces or polymers with fluorescent properties for sensor development.

Basic Information

Item	Detail
Product Name	Trisulfo-Cy5-Alkyne
CAS No.	2055138-90-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5 Alkyne, trisulfonated; Sulfo-Cyanine5 Alkyne; Trisulfonated Cy5 Alkyne; 1,1'-(4,7,10-Trioxa-1,13-tridecanediyl)bis[1H-pyrrole-2,5-dione] derivative; NIR fluorescent alkyne dye; Water-soluble Cy5 alkyne; Click chemistry Cy5 dye
EINECS	Contact for details

Quality Control

Our Trisulfo-Cy5-Alkyne is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and absorbance/fluorescence spectroscopy for performance validation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Absorption Max (λmax)	~649 nm
Emission Max (λem)	~670 nm
Solubility	Freely soluble in water, DMSO, PBS
Moisture Content	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.