Description

Disulfo-Cy5 Azide is a water-soluble, near-infrared fluorescent labeling reagent featuring a reactive azide group for bioorthogonal click chemistry. This compound is essential for researchers requiring stable, high-performance fluorescent tags with minimal background interference in aqueous biological systems. It is primarily needed by professionals in bioconjugation, proteomics, and in vivo imaging for labeling biomolecules such as antibodies, proteins, and oligonucleotides.

Application

• Bioconjugation and Probe Development: Site-specific labeling of proteins, antibodies, and other biomolecules via copper-free click chemistry with alkynes.
• Bioimaging and Diagnostics: Fluorescent tagging for in vitro and in vivo near-infrared (NIR) imaging applications, offering deeper tissue penetration.
• Proteomics and Biomarker Discovery: Used in activity-based protein profiling (ABPP) and other fluorescence-based detection assays.
• Nucleic Acid Labeling: Conjugation to modified oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Material Science: Surface functionalization of nanoparticles and polymers for creating fluorescent nanomaterials.
• Flow Cytometry and Cell Sorting: Development of fluorescent antibodies and other cell surface markers for high-throughput analysis.

Basic Information

Item	Detail
Product Name	Disulfo-Cy5 Azide
CAS No.	2055138-89-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cyanine5 azide, disulfonated; Sulfo-Cy5 N3; Disulfo-Cyanine5 Azide; Cy5 azide, water-soluble; Azide-functionalized Cy5 dye; Near-IR fluorescent azide; 2055138-89-9; CAS 2055138-89-9
EINECS	Contact for details

Quality Control

Our Disulfo-Cy5 Azide is manufactured under strict quality protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and absorbance/fluorescence spectroscopy to ensure performance specifications are met. Certificates of Analysis (COA) detailing all test results are available upon request to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Absorption Maximum (λmax)	~649 nm
Emission Maximum (λem)	~670 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in water, DMSO, PBS
Moisture Content (KF)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.