Description

Disulfo-Cy3 Alkyne CAS NO 2055138-88-8 is a water-soluble, near-infrared fluorescent labeling reagent featuring a terminal alkyne group for bioorthogonal click chemistry conjugation. This compound is engineered for superior performance in demanding biological imaging applications, offering enhanced brightness and photostability while minimizing non-specific binding. It is an essential tool for researchers and developers in bioconjugation, live-cell imaging, and in vivo diagnostics, enabling the precise tagging and tracking of biomolecules.

Application

• Bioorthogonal Labeling: Conjugation to azide-modified biomolecules (proteins, antibodies, nucleic acids) via copper-catalyzed or copper-free click chemistry.
• Live-Cell and Super-Resolution Imaging: Long-term tracking of cellular processes due to excellent photostability and reduced phototoxicity.
• In Vivo Fluorescence Imaging: NIR emission allows for deeper tissue penetration with minimal background autofluorescence in animal studies.
• Flow Cytometry & Microscopy: High-sensitivity detection and analysis of labeled cells and subcellular structures.
• Diagnostic Assay Development: Used as a fluorescent reporter in ELISA, lateral flow assays, and other diagnostic platforms.
• Polymer and Material Science: Fluorescent tagging of synthetic polymers, nanoparticles, and hydrogels for characterization and tracking.

Basic Information

Product Name	Disulfo-Cy3 Alkyne
CAS No.	2055138-88-8
Molecular Formula	C37H41KN2O10S2 (Potassium salt form)
Molecular Weight	~801.0 g/mol
Synonyms	Sulfo-Cyanine3 alkyne; Cy3® alkyne, disulfonated; 2-((1E,3E)-5-((E)-1-(5-(Hex-5-yn-1-yl)-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)indolin-2-ylidene)ethylidene)-4-((E)-2-((E)-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)indolin-2-ylidene)ethylidene)cyclopent-1-en-1-yl)-1-(5-(hex-5-yn-1-yl)-3,3-dimethyl-1-(3-sulfopropyl)-3H-indol-1-ium-5-yl)ethan-1-ide; Potassium 1-(5-(hex-5-yn-1-yl)-3,3-dimethyl-1-(3-sulfonatopropyl)indolin-2-ylidene)-2-((1E,3E)-5-((E)-1-(5-(hex-5-yn-1-yl)-3,3-dimethyl-1-(3-sulfonatopropyl)indolin-2-ylidene)ethylidene)-4-((E)-2-((E)-3,3-dimethyl-1-(3-sulfonatopropyl)indolin-2-ylidene)ethylidene)cyclopent-1-en-1-yl)vinyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
EINECS	Contact for details

Quality Control

Our Disulfo-Cy3 Alkyne is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and MS for identity verification, to ensure consistent performance and batch-to-batch reproducibility. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Absorption (λmax)	548 - 554 nm
Emission (λmax)	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Solubility	Freely soluble in water, DMSO, PBS

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.