Description

Sulfo-Cy3-Tetrazine CAS NO 2055138-86-6 is a water-soluble, near-infrared fluorescent dye conjugate designed for bioorthogonal labeling and imaging applications. This compound is engineered to enable highly specific, rapid, and biocompatible click chemistry reactions, primarily with trans-cyclooctene (TCO) moieties. It is an essential tool for researchers and developers in the fields of bioconjugation, live-cell imaging, and in vivo diagnostics, providing superior brightness and photostability.

Application

• Bioorthogonal Labeling: For the specific tagging of biomolecules (e.g., antibodies, proteins, peptides) via tetrazine ligation with TCO-modified targets.
• Live-Cell Imaging: Enables real-time visualization of cellular processes and dynamics with minimal background fluorescence and high target specificity.
• In Vivo Imaging: Used in preclinical research for fluorescence-guided surgery and tracking of targeted therapeutics in animal models.
• Diagnostic Probe Development: Serves as a key component in the design of fluorescent probes and assays for diagnostic applications.
• Flow Cytometry: Facilitates high-throughput cell sorting and analysis when conjugated to targeting ligands.
• Super-Resolution Microscopy: Provides the photophysical properties required for advanced imaging techniques like STORM and PALM.

Basic Information

Product Name	Sulfo-Cy3-Tetrazine
CAS No.	2055138-86-6
Molecular Formula	C41H45N7O8S22- • 2Na+
Molecular Weight	859.94 g/mol (Free acid)
Synonyms	Sulfo-Cyanine3-Tetrazine; Sulfo Cy3 TZ; Cyanine3-sulfo-tetrazine; 1-(6-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-5-((E)-1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium-5-sulfonate; Sulfo-Cy3-TCO Click Chemistry Dye; Water-soluble Cy3-Tetrazine; Bioorthogonal Cy3 dye
EINECS	Contact for details

Quality Control

Our Sulfo-Cy3-Tetrazine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and performance metrics are available upon request to ensure it meets the rigorous standards required for life science research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate and keep frozen. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~150,000 cm-1M-1 (at λmax)
Fluorescence Emission (λem)	~570 nm
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.