Description

Fluorescein-Peg6-Bis-Nhs Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for creating stable, fluorescently labeled biomolecules with precise control over linker length and functionality. It is primarily utilized by researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and advanced materials science who require reliable and efficient labeling strategies.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to antibodies via amine groups, incorporating a fluorescent reporter for tracking.
• Protein and Peptide Labeling: Fluorescent tagging of proteins, enzymes, and peptides for fluorescence microscopy, flow cytometry (FACS), and cellular imaging studies.
• Diagnostic Probe Synthesis: Construction of fluorescent probes and biosensors for in vitro diagnostics (IVD) and high-throughput screening (HTS) applications.
• Nanoparticle Functionalization: Surface modification of liposomes, polymeric nanoparticles, and quantum dots to create targeted, traceable delivery systems.
• Biomaterial and Hydrogel Crosslinking: Incorporating fluorescent markers into polymer networks for degradation and biodistribution studies in tissue engineering.
• Oligonucleotide Conjugation: Labeling of DNA, RNA, or siRNA with fluorescein for genetic analysis, fluorescence in situ hybridization (FISH), and therapeutic monitoring.

Basic Information

Product Name	Fluorescein-Peg6-Bis-Nhs Ester
CAS No.	2055105-59-2
Molecular Formula	C52H62N4O21
Molecular Weight	1079.07 g/mol
Synonyms	5(6)-Fluorescein-PEG6-Bis-NHS ester; Fluorescein-PEG6-NHS ester bis-succinimidyl; 6-(Fluorescein-5(6)-carboxamido)hexanoic acid, 2,5-dioxo-1-pyrrolidinyl ester PEGylated; 5/6-FAM-PEG6-Bis-NHS; Fluorescein-PEG6-Bis(N-succinimidyl) Succinate; 5(6)-Carboxyfluorescein-PEG6-Bis-SC; Heterobifunctional PEG linker, Fluorescein and NHS terminated; Nα-(5/6-Carboxyfluorescein)-PEG6-NHS ester
EINECS	Contact for details

Quality Control

Our Fluorescein-Peg6-Bis-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.