Description

Trisulfo-Cy5.5-Alkyne is a near-infrared (NIR) fluorescent dye derivative functionalized with an alkyne group for bioorthogonal click chemistry. This compound is engineered for high-performance labeling and detection in complex biological systems, offering superior water solubility and photostability due to its trisulfonated structure. It is an essential reagent for researchers and developers in bioconjugation, in vivo imaging, and diagnostic assay development, providing a critical tool for sensitive and specific target visualization.

Application

• Bioorthogonal Labeling: Conjugation to azide-modified biomolecules (proteins, antibodies, nucleic acids) via copper-catalyzed or copper-free click chemistry for the creation of NIR fluorescent probes.
• In Vivo Imaging: Labeling of cells, antibodies, or nanoparticles for deep-tissue fluorescence imaging in preclinical animal studies, leveraging the minimal tissue autofluorescence in the NIR window.
• Diagnostic Assay Development: Used as a fluorescent tag in lateral flow assays (LFAs), enzyme-linked immunosorbent assays (ELISAs), and other immunoassays to enhance detection sensitivity.
• Flow Cytometry & Microscopy: Staining and tracking of specific cell populations or intracellular targets in flow cytometry and fluorescence microscopy applications.
• Drug Delivery & Theranostics: Conjugation to drug carriers or therapeutic agents to enable simultaneous tracking of biodistribution and therapeutic efficacy.
• Material Science: Functionalization of polymers or surfaces with NIR fluorescence properties for sensor development or advanced material tracking.

Basic Information

Product Name	Trisulfo-Cy5.5-Alkyne
CAS No.	2055046-12-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5.5 Alkyne, Trisulfonated; Sulfo-Cyanine5.5 Alkyne; Trisulfonated Cy5.5 Alkyne; NIR Dye Alkyne, Trisulfo-Cy5.5; Click Chemistry Cy5.5 Dye; Water-soluble Cy5.5 Alkyne; 2055046-12-1
EINECS	Contact for details

Quality Control

Our Trisulfo-Cy5.5-Alkyne is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. A Certificate of Analysis (COA) detailing lot-specific results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~ 675 nm
Emission (λmax)	~ 695 nm
Moisture Content (KF)	≤ 5.0 %
Solubility	Freely soluble in water, DMSO, PBS

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.