Description

n-(Azido-Peg3)-n-Bis-(Peg3-Nhs Ester) is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and two NHS ester termini. This compound is engineered for efficient and selective bioconjugation, enabling the stable linkage of biomolecules under mild, aqueous conditions. It is essential for researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and advanced material science who require precise and reliable crosslinking strategies.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via click chemistry and amine coupling.
• PEGylation of Proteins and Peptides: To enhance pharmacokinetic properties such as solubility, stability, and circulation half-life.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray slides with bioactive ligands.
• Hydrogel Formation: As a crosslinking agent in the synthesis of biocompatible PEG-based hydrogels for tissue engineering and drug delivery.
• Diagnostic Reagent Synthesis: In the creation of labeled probes and detection complexes for immunoassays and molecular imaging.
• Polymer Chemistry: As a building block for synthesizing functionalized, multi-arm PEG polymers with controlled architectures.

Basic Information

Product Name	n-(Azido-Peg3)-n-Bis-(Peg3-Nhs Ester)
CAS No.	2055042-58-3
Molecular Formula	C38H60N4O18
Molecular Weight	860.9 g/mol
Synonyms	Azido-PEG3-tris-NHS ester; NHS-PEG3-Azide-PEG3-NHS; 1-Azido-3,6,9,12,15,18,21,24,27-nonaoxanonacosane-29,29-diyl bis(2,5-dioxo-1-pyrrolidinecarboxylate); N3-(PEG3)-N,N-bis(PEG3-NHS ester); Azido-PEG3-tris(PEG3-NHS ester); Heterobifunctional PEG crosslinker, Azide/NHS; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]-3-oxo-3-[(2,5-dioxopyrrolidin-1-yl)oxy]propoxy]ethoxy]ethoxy]ethoxy]propanoate
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Bis-(Peg3-Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group analysis, to ensure consistency and performance. Certificates of Analysis (COA) with detailed specifications are provided to support your research and cGMP-compliant production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 90% of theoretical
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.