Description

Azido-Peg3-S-Peg4-T-Butyl Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and a protected carboxylic acid. This compound is engineered to facilitate controlled bioconjugation and click chemistry reactions, enabling precise modification of biomolecules, surfaces, and polymers. It is a critical reagent for researchers and manufacturers in the fields of biopharmaceutical development, advanced material science, and diagnostic probe synthesis, where reliable, high-purity linkers are essential for product performance.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Used as a key linker to attach cytotoxic payloads to monoclonal antibodies via strain-promoted azide-alkyne cycloaddition (SPAAC) or copper-catalyzed click chemistry.
• PEGylation and Protein Modification: Enables site-specific PEGylation of peptides and proteins to enhance solubility, stability, and circulation half-life.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray slides with bioactive ligands for targeted delivery and diagnostic applications.
• Polymer and Hydrogel Synthesis: Serves as a functional crosslinker in the creation of smart, biodegradable hydrogels for drug delivery and tissue engineering.
• Diagnostic Reagent Manufacturing: Used in the synthesis of fluorescent probes, imaging agents, and other diagnostic tools requiring stable, water-soluble linkers.
• Chemical Biology and Proteomics: A versatile tool for labeling, pull-down assays, and activity-based protein profiling (ABPP) studies.

Basic Information

Product Name	Azido-Peg3-S-Peg4-T-Butyl Ester
CAS No.	2055041-19-3
Molecular Formula	C28H53N3O11S
Molecular Weight	615.8 g/mol
Synonyms	Azido-PEG3-S-PEG4-t-butyl ester; 3-Azidopropyl-[2-[2-[2-[2-[2-[2-[2-(2-tert-butoxy-2-oxoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl] sulfide; 1-tert-Butyl 17-(3-azidopropylsulfanyl)-3,6,9,12,15-pentaoxaheptadecane-1,17-dioate; Azido-PEG3-SS-PEG4-COOtBu (common abbreviation); Heterobifunctional PEG crosslinker, Azide-PEG-SS-PEG-tBu ester; N3-PEG3-S-PEG4-OtBu; Azido-PEG3-Thiol-PEG4-t-Butyl Ester
EINECS	Contact for details

Quality Control

Our Azido-Peg3-S-Peg4-T-Butyl Ester is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive research and GMP-grade applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.