Description

Azido-Peg4-Val-Cit-Pab-Oh (CAS NO 2055024-64-9) is a high-purity, heterobifunctional linker compound designed for advanced bioconjugation and drug delivery applications. Its core value lies in its precise structure, which incorporates a cleavable Valine-Citrulline (Val-Cit) dipeptide sequence, a self-immolative para-aminobenzyloxycarbonyl (PABC) spacer, and a terminal azide group for bioorthogonal click chemistry. This product is essential for researchers and manufacturers in the fields of antibody-drug conjugate (ADC) development, targeted therapeutics, and proteomics. It enables the stable, site-specific attachment of cytotoxic payloads to targeting molecules, a critical step in creating next-generation precision medicines.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a key component in the synthesis of ADCs, utilizing its protease-cleavable Val-Cit-PABC motif for controlled drug release in tumor microenvironments.
• Bioconjugation and Click Chemistry: The terminal azide group enables efficient, copper-catalyzed or strain-promoted azide-alkyne cycloaddition (SPAAC) with alkyne-functionalized molecules, peptides, or surfaces.
• Prodrug Synthesis: Used in the design and construction of prodrugs where a therapeutic agent is masked by the linker and selectively activated by specific enzymes like cathepsin B.
• Polymer-Drug Conjugates: Facilitates the creation of advanced polymer therapeutics by linking drug molecules to polyethylene glycol (PEG) or other polymeric carriers via the cleavable linker.
• Diagnostic Agent Development: Applied in the conjugation of imaging agents (e.g., fluorophores, radiolabels) to targeting antibodies or peptides for in vivo diagnostics.
• Solid-Phase Peptide Synthesis (SPPS): Acts as a specialized building block for introducing cleavable linkers and functional handles during peptide synthesis.
• Chemical Biology Tool: A valuable reagent for studying enzyme activity, intracellular trafficking, and developing novel bioassays.

Basic Information

Product Name	Azido-Peg4-Val-Cit-Pab-Oh
CAS No.	2055024-64-9
Molecular Formula	C36H58N8O13
Molecular Weight	786.90 g/mol
Synonyms	Azido-PEG4-Val-Cit-PAB-OH; N3-PEG4-VC-PAB-OH; Azido-PEG4-Val-Cit-PABC-OH; 11-Azido-3,6,9-trioxaundecan-1-oyl-Valine-Citrulline-p-aminobenzyl alcohol; ADC Linker Azido-PEG4-VC-PAB; 1-Azido-3,6,9,12-tetraoxapentadecan-15-oic acid, 15-[[(2S)-5-amino-1-[[(2S)-1-[[4-[[(2S)-1-[[(2S)-1-[[(2S,3R)-1-[[(1S)-2-methyl-1-[[(2S)-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1-oxobutan-2-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopropan-2-yl]amino]phenyl]methoxy]carbonyl]amino]-1-oxopentan-2-yl]amino]-15-oxo-; Azido-PEG4-VC-PABC-OH
EINECS	Contact for details

Quality Control

Our Azido-Peg4-Val-Cit-Pab-Oh is manufactured under strict quality management systems to ensure the high purity and consistency required for sensitive bioconjugation research and cGMP-grade ADC manufacturing. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, NMR for structural verification, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting the results against our stringent internal specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere (argon or nitrogen). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.