Description

Fluorescein-Dbco CAS NO 2054339-00-1 is a high-purity chemical reagent that combines the bright green fluorescence of fluorescein with the bioorthogonal reactivity of a dibenzocyclooctyne (DBCO) group. This bifunctional molecule is critical for enabling strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry in complex biological environments without the need for cytotoxic copper catalysts. It is an essential tool for researchers and developers in bioconjugation, bioimaging, and diagnostic assay development, allowing for the specific, covalent labeling of azide-modified biomolecules such as proteins, antibodies, and oligonucleotides with a fluorescent tag.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to azide-functionalized proteins, peptides, antibodies, and nucleic acids for tracking and detection.
• Live-Cell and In Vivo Imaging: Used in SPAAC click chemistry for real-time visualization of cellular processes and biodistribution studies due to its excellent fluorescence properties and bioorthogonality.
• Diagnostic Assay Development: Serves as a key component in the development of fluorescent immunoassays, lateral flow tests, and other diagnostic platforms.
• Polymer and Materials Science: Functionalization of azide-containing polymers, hydrogels, and nanoparticles to introduce fluorescent properties for material tracking and characterization.
• Proteomics and Biomarker Discovery: Labeling of azide-tagged metabolites or glycans for fluorescence-based analysis and separation techniques.
• Antibody-Drug Conjugate (ADC) Research: A model fluorescent payload for developing and optimizing novel bioconjugation strategies in ADC linker technology.

Basic Information

Product Name	Fluorescein-Dbco
CAS No.	2054339-00-1
Molecular Formula	C37H20N2O7
Molecular Weight	604.57 g/mol
Synonyms	5-Isomer Fluorescein-DBCO; DBCO-Fluorescein; DBCO-FL; 5-DBCO-Fluorescein; Dibenzocyclooctyne-Fluorescein; 5-Carboxyfluorescein-DBCO; 6-((6-((3aS,4S,6aR)-2,3,3a,4,6,6a-Hexahydro-1H-cyclopenta[c]furan-4-yloxy)hexyl)amino)-6-oxohexyl 6-(3-oxo-3H-xanthen-9-yl)-5-((3aS,4S,6aR)-2-oxo-1,2,3,3a,4,6a-hexahydrocyclopenta[c]furan-4-yloxy)hexanoate (IUPAC); Azadibenzocyclooctyne Fluorescein Conjugate
EINECS	Contact for details

Quality Control

Our Fluorescein-Dbco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~494 nm
Emission (λmax)	~518 nm
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.