Description

Cyanine5.5 NHS Ester is a near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules via primary amine groups. This compound is critical for researchers and developers requiring high-sensitivity detection and imaging in complex biological systems. It is primarily utilized in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as antibody labeling, protein tracking, and in vivo imaging.

Application

• Antibody-Dye Conjugate Preparation: For creating fluorescently labeled antibodies used in flow cytometry, immunohistochemistry (IHC), and Western blotting.
• Protein and Peptide Labeling: Enables the tagging of proteins and peptides for tracking, purification, and interaction studies.
• Nucleic Acid Probes: Used in the synthesis of fluorescent probes for fluorescence in situ hybridization (FISH) and microarray analysis.
• In Vivo Imaging Agents: A key component in developing contrast agents for non-invasive near-infrared fluorescence (NIRF) imaging in preclinical research.
• Diagnostic Assay Development: Incorporated into lateral flow assays, ELISA, and other diagnostic platforms for sensitive detection.
• Polymer and Nanoparticle Functionalization: For attaching fluorescent markers to polymers, dendrimers, and nanoparticles for drug delivery tracking.

Basic Information

Product Name	Cyanine5.5 NHS Ester
CAS No.	2054318-82-8
Molecular Formula	C44H49N3O6
Molecular Weight	715.89 g/mol
Synonyms	Cy5.5 NHS Ester; Cy5.5 N-Hydroxysuccinimide Ester; Sulfo-Cyanine5.5 NHS Ester (if sulfonated); 1-(5-Carboxypentyl)-1'-ethyl-3,3,3',3'-tetramethyl-4,5,4',5'-dibenzo-2,2'-indotricarbocyanine N-hydroxysuccinimide ester; 2,5-Dioxopyrrolidin-1-yl (E)-3-(2-((E)-1-ethyl-3,3-dimethyl-5-sulfo-3H-indol-1-ium-2-yl)vinyl)-5-((E)-2-((E)-3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxoprop-1-en-1-yl)-5,5-dimethylcyclohex-1-en-1-yl)penta-1,3-dien-1-yl)-5,5-dimethyl-1-sulfo-3H-indol-1-ium-3-yl)prop-2-enoate; Cy™5.5 NHS Ester; NIR Dye NHS Ester
EINECS	Contact for details

Quality Control

Our Cyanine5.5 NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~675 - 685 nm
Emission Maximum (λem)	~695 - 705 nm
Extinction Coefficient (ε)	≥ 190,000 cm⁻¹M⁻¹
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.