Description

5(6)-Carboxyfluorescein Diisobutyrate is a chemically modified, esterified derivative of the widely used fluorescent dye carboxyfluorescein. This compound is specifically engineered to offer enhanced cell permeability and stability, making it a critical reagent for advanced biological and biochemical research. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic sectors for applications requiring precise intracellular labeling and tracking.

Application

• Intracellular pH Sensing and Measurement: Acts as a fluorescent probe for monitoring pH changes within live cells due to its pH-dependent fluorescence.
• Cell Viability and Cytotoxicity Assays: Used to assess cell membrane integrity and metabolic activity in high-throughput screening.
• Fluorescent Tracer and Marker: Employed in cell migration studies, phagocytosis assays, and fluid-phase endocytosis tracking.
• Drug Delivery Research: Serves as a model compound or a fluorescent tag to study the uptake and release kinetics of drug delivery systems.
• Enzyme Activity Assays: Functions as a substrate for esterase enzymes, where cleavage releases highly fluorescent carboxyfluorescein, enabling kinetic studies.
• Diagnostic Kit Development: Incorporated as a key component in the development of fluorescent-based diagnostic tests and biosensors.

Basic Information

Product Name	5(6)-Carboxyfluorescein Diisobutyrate
CAS No.	287111-44-8
Molecular Formula	C29H26O9
Molecular Weight	518.51 g/mol
Synonyms	5(6)-CFDA Diisobutyrate; 5(6)-Carboxyfluorescein Diacetate Diisobutyrate (mixed isomers); CFDA-IB; 3',6'-Bis(isobutyryloxy)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic Acid; Fluorescein Diisobutyrate 5(6)-Carboxy; 5(6)-Carboxy-2',7'-dichlorofluorescein Diisobutyrate derivative (common base structure); 5(6)-FAM Diisobutyrate (related analog)
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxyfluorescein Diisobutyrate is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results is provided with every shipment to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (typical ≥ 97.0%)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.