Description

7-Iodoacetamidocoumarin-4-Carboxylic Acid is a highly functionalized coumarin derivative that serves as a critical building block and fluorescent probe in advanced chemical synthesis. Its core value lies in the reactive iodoacetamide group, which enables selective conjugation with thiols, and the carboxylic acid moiety, which facilitates further derivatization or immobilization. This bifunctional reagent is essential for researchers and manufacturers in pharmaceutical development, bioconjugation, and materials science, where precise labeling and modification of biomolecules are required.

Application

• Bioconjugation and Protein Labeling: The iodoacetamide group selectively reacts with cysteine thiols in proteins and peptides, while the coumarin fluorophore provides a sensitive readout, making it ideal for creating fluorescent protein conjugates.
• Fluorescent Probe Synthesis: Acts as a key precursor for developing site-specific fluorescent sensors and activity-based probes for detecting enzymatic activity or cellular localization.
• Pharmaceutical Intermediate: Used in the research and development of novel drug candidates, particularly those targeting specific biological pathways where fluorescence tracking is beneficial.
• Biomaterial Functionalization: Employed to introduce fluorescent tags onto polymer surfaces, nanoparticles, or hydrogels for tracking and imaging applications in biomedical engineering.
• Chemical Biology Research: A vital tool for studying protein-protein interactions, dynamics, and folding through fluorescence resonance energy transfer (FRET) or other spectroscopic techniques.
• Diagnostic Reagent Development: Utilized in the creation of components for diagnostic assays and kits that require high-sensitivity fluorescence detection.

Basic Information

Product Name	7-Iodoacetamidocoumarin-4-Carboxylic Acid
CAS No.	284679-24-9
Molecular Formula	C₁₄H₉INO₅
Molecular Weight	398.13 g/mol
Synonyms	4-Carboxy-7-iodoacetamidocoumarin; 7-[(Iodoacetyl)amino]-4-carboxycoumarin; 7-Iodoacetamido-4-carboxycoumarin; Iodoacetamidocoumarin carboxylic acid; Coumarin IA-4-COOH; 4-Carboxy-7-IA-coumarin; Fluorescent thiol-reactive probe; 7-IA-4-CCA
EINECS	Contact for details

Quality Control

Our 7-Iodoacetamidocoumarin-4-Carboxylic Acid is manufactured under strict quality protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients in regulated development pathways.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light may lead to decomposition. For long-term storage, consider desiccation and under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.