Description

Fluo-4-Am For Fluorescence Pge. With 5 is a high-purity, cell-permeant fluorescent calcium indicator dye, essential for real-time intracellular calcium ion (Ca²⁺) concentration measurement. Its core value lies in its high affinity for calcium, resulting in a significant fluorescence intensity increase upon binding, enabling precise quantitative analysis in live-cell imaging. This product is critically needed by researchers and quality control laboratories in the life sciences, pharmaceutical discovery, and biotechnology sectors for applications in cell signaling studies, drug screening, and neurobiological research.

Application

• Live-Cell Calcium Imaging: Primary tool for visualizing and quantifying dynamic changes in intracellular Ca²⁺ levels in neurons, cardiomyocytes, and other cell types.
• Drug Discovery & High-Throughput Screening (HTS): Used in assays to evaluate the effect of pharmacological compounds on calcium signaling pathways and GPCR activity.
• Neuroscience Research: Critical for studying synaptic transmission, neuronal excitability, and calcium-dependent processes in brain function and disease models.
• Cardiovascular Research: Monitoring calcium transients in cardiac muscle cells to study contractility, arrhythmias, and cardiotoxicity.
• Cell Biology & Physiology: Investigating calcium's role as a second messenger in processes like muscle contraction, secretion, gene expression, and apoptosis.
• FLIPR (Fluorometric Imaging Plate Reader) Assays: A standard dye for fluorescence-based plate reader systems in automated screening environments.

Basic Information

Item	Detail
Product Name	Fluo-4-Am For Fluorescence Pge. With 5
CAS No.	273221-67-3
Molecular Formula	C₅₁H₅₀F₂N₂O₂₃
Molecular Weight	1096.93 g/mol
Synonyms	Fluo-4 AM; Fluo-4 Acetoxymethyl Ester; Fluo-4, AM ester; Fluo-4 pentapotassium salt, cell permeant; 2-[2-[2-[2-[Bis(carboxymethyl)amino]-5-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylphenyl]-1,3,2-dioxaborinane-4,6-dione Acetoxymethyl Ester; F-14201; Calcium indicator Fluo-4 AM
EINECS	Contact for details

Quality Control

Every batch of Fluo-4-Am is manufactured and tested under strict quality management systems to ensure the highest purity and performance consistency required for sensitive research applications. Our quality protocols include rigorous analytical testing by HPLC and spectroscopic methods to confirm identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to guarantee traceability and compliance with your research standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~494/516 nm (Ca²⁺ bound)
Functional Performance (In-cell assay)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.