Description

Hoechst 33258 Analog CAS NO 258843-62-8 is a high-purity, synthetic derivative of the classic bis-benzimide DNA stain Hoechst 33258. This compound is valued for its specific binding affinity to adenine-thymine (A-T) rich regions of double-stranded DNA, making it an essential tool for advanced molecular biology and cytogenetics research. It is primarily utilized by researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic sectors for applications requiring precise nucleic acid detection and quantification.

Application

• Fluorescent DNA Staining: Used in fluorescence microscopy and flow cytometry for visualizing nuclear DNA in fixed cells and chromosomes.
• Quantitative DNA Analysis: Employed in fluorometric assays for the precise quantification of double-stranded DNA content in solution.
• Cell Viability/Proliferation Assays: Acts as a nuclear counterstain in multiplex assays to distinguish live/dead cells or to quantify cell numbers.
• Chromosome Banding (Cytogenetics): Useful in techniques for chromosome identification and karyotyping due to its specific A-T binding preference.
• High-Content Screening (HCS): Integrated into automated imaging platforms for drug discovery and toxicology studies that require nuclear segmentation and analysis.
• Research on DNA Structure & Dynamics: Serves as a probe in biophysical studies investigating DNA-ligand interactions, chromatin organization, and DNA damage.

Basic Information

Product Name	Hoechst 33258 Analog
CAS No.	258843-62-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Hoechst 33258 derivative; Bisbenzimide H 33258 analog; Phenylindole derivative; DNA stain 33258 analog; AT-selective DNA minor groove binder; Fluorochrome 33258 analog; Research dye 258843-62-8; Fluorescent probe for dsDNA
EINECS	Contact for details

Quality Control

Every batch of Hoechst 33258 Analog is manufactured and tested under strict quality management systems to ensure consistency and performance for critical research applications. Our products undergo rigorous quality testing, including spectroscopic purity analysis and functional performance checks, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and spectral properties are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a dry environment. For long-term stability, consider storage at 2-8°C. Keep the container sealed to protect from moisture.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Fluorescence Properties	Excitation/Emission maxima consistent with reference
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.