Description

C12 Nbd-Sphingomyelin is a fluorescently labeled sphingolipid derivative, specifically designed for advanced lipid research and cellular imaging. This compound is critical for studying membrane dynamics, lipid trafficking, and sphingomyelin metabolism in live cells with high sensitivity. It is an essential tool for researchers and scientists in the fields of biochemistry, cell biology, and pharmaceutical development who require precise, reliable probes for fluorescence microscopy and spectroscopy.

Application

• Live-Cell Imaging: Used as a fluorescent tracer to visualize the distribution and movement of sphingomyelin in real-time within cellular membranes.
• Lipid Raft & Membrane Dynamics Studies: Investigates the role of sphingomyelin in lipid raft formation, membrane fluidity, and microdomain organization.
• Intracellular Trafficking Assays: Tracks the endocytic and secretory pathways of sphingolipids to understand cellular transport mechanisms.
• Sphingolipid Metabolism Research: Serves as a substrate or probe in enzymatic assays to study sphingomyelinases and other metabolic enzymes.
• Model Membrane Systems: Incorporated into liposomes or supported lipid bilayers (SLBs) for in vitro biophysical studies of membrane properties.
• Drug Discovery & Screening: Utilized in high-content screening (HCS) platforms to identify compounds that affect sphingolipid pathways or membrane integrity.
• Apoptosis & Cell Signaling Research: Helps elucidate the involvement of sphingomyelin and its metabolites in cell death and signal transduction pathways.

Basic Information

Product Name	C12 Nbd-Sphingomyelin
CAS No.	254117-01-6
Molecular Formula	C47H86N5O11P
Molecular Weight	928.19 g/mol
Synonyms	NBD C12-Sphingomyelin; N-(12-[(7-Nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl)sphingosylphosphorylcholine; 1-Oleoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphocholine (related analog); Fluorescent Sphingomyelin C12; NBD-labeled Sphingomyelin; SM-NBD C12; Sphingomyelin analog, NBD C12; Lipid probe NBD-SM
EINECS	Contact for details

Quality Control

Every batch of C12 Nbd-Sphingomyelin is manufactured and analyzed under strict quality protocols to ensure the highest standards of purity and performance for research applications. Our products undergo rigorous quality testing, including HPLC for purity and spectroscopic methods for identity confirmation, to ensure batch-to-batch consistency and compliance with research-grade specifications. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the container sealed under an inert atmosphere (e.g., argon or nitrogen) to prevent degradation. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in chloroform, methanol, DMSO
Extinction Coefficient (ε)	~21,000 cm-1M-1 at ~465 nm (in methanol)
Fluorescence Emission	~535 nm (green)
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.