Description

Draq5 is a far-red fluorescent DNA dye widely used as a cell-permeant nuclear stain for live-cell imaging. Its core value proposition lies in its high photostability and compatibility with common laser lines, enabling long-term tracking of cellular processes without significant photobleaching. This product is essential for researchers and professionals in cell biology, high-content screening, and flow cytometry who require reliable and specific nuclear staining in viable cells.

Application

• Live-Cell Nuclear Staining: For real-time visualization and tracking of nuclei in living cells over extended periods.
• High-Content Screening (HCS): Used in automated imaging platforms for cell-based assays to quantify nuclear morphology, count, and cell cycle status.
• Flow Cytometry: Employed for DNA content analysis and cell cycle profiling in live cell populations.
• Multiplex Fluorescence Imaging: Ideal for co-staining with green (e.g., GFP) and orange/red fluorescent proteins or dyes due to its far-red emission.
• Long-Term Time-Lapse Microscopy: Facilitates studies of cell division, migration, and apoptosis thanks to its exceptional photostability.
• 3D Cell Culture & Spheroid Imaging: Penetrates deeper into tissue samples for clear nuclear visualization in complex structures.

Basic Information

Product Name	Draq5
CAS No.	254098-36-7
Molecular Formula	C₃₃H₃₉N₅O₃
Molecular Weight	553.70 g/mol
Synonyms	DRAQ5; DRAQ5™; Benzo[cd]indol-2(1H)-one, 1-[3-(dimethylamino)propyl]-1,3-dihydro-3,3-dimethyl-5-[4-(methylamino)-1-naphthalenyl]; Far-red DNA dye; Cell-impermeant nuclear dye; Anthraquinone dye derivative; Live cell DNA stain
EINECS	Contact for details

Quality Control

Every batch of Draq5 is manufactured and tested under strict quality management systems to ensure high purity, consistent performance, and batch-to-batch reproducibility critical for research integrity. Our quality control includes rigorous analytical testing by HPLC and spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark blue to violet solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV-Vis)	Spectrum matches reference
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~646 nm (in methanol)
Emission Maximum	~681 nm (in methanol)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Performance	Validated for live-cell nuclear staining

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.