Description

Cyanine 5 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt is a reactive, near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This compound is essential for creating stable, bright fluorescent probes for advanced bioanalytical applications. It is primarily used by researchers and manufacturers in the fields of bioconjugation, flow cytometry, in vivo imaging, and diagnostic assay development.

Application

• Antibody and Protein Labeling: Conjugation to primary amines for use in immunofluorescence, Western blotting, and ELISA.
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• In Vivo Imaging: Preparation of targeted imaging agents for preclinical small animal studies due to its favorable near-infrared emission.
• Flow Cytometry: Creation of bright, photostable fluorescent conjugates for cell surface marker analysis.
• Microarray and Biosensor Development: Immobilization of labeled biomolecules on solid surfaces for high-throughput screening.
• Drug Delivery Research: Tracking and visualization of nanoparticle or therapeutic conjugates.

Basic Information

Product Name	Cyanine 5 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt
CAS No.	252255-42-8
Molecular Formula	C41H51KN2O8
Molecular Weight	738.95 g/mol
Synonyms	Cy5 Bihexanoic Acid, Succinimidyl Ester, Potassium Salt; Cy5 NHS Ester, Potassium Salt; Sulfo-Cy5 NHS Ester (alternative naming); Cyanine5.18 Bihexanoic Acid, N-Succinimidyl Ester, Monopotassium Salt; 1,1'-(1E,1'E)-((6,6'-(Hexane-1,6-diylbis(oxy))bis(hexane-6,1-diyl))bis(ethene-2,1-diyl))bis(2,3,3-trimethyl-3H-indol-1-ium-5-carboxylate) bis(N-succinimidyl) ester, dipotassium salt
EINECS	Contact for details

Quality Control

Our Cyanine 5 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and batch-to-batch reproducibility. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~250,000 L·mol⁻¹·cm⁻¹ (at λmax ~646 nm in methanol)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF, acetonitrile; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.