Description

Cyanine 5 Bihexanoic Acid Dye, Potassium Salt is a near-infrared fluorescent dye derivative designed for superior aqueous solubility and labeling efficiency. This compound is a critical reagent for advanced bioimaging and diagnostic applications, offering bright, stable fluorescence with minimal background interference. It is primarily needed by researchers and manufacturers in the biotechnology, pharmaceutical, and in vitro diagnostics sectors for developing sensitive assays, flow cytometry panels, and in vivo imaging probes.

Application

• Bioconjugation and Probe Development: Labeling of antibodies, proteins, peptides, and oligonucleotides for fluorescence-based detection systems.
• In Vivo and In Vitro Imaging: Used in the development of contrast agents for near-infrared fluorescence (NIRF) imaging in preclinical research.
• Flow Cytometry & Microscopy: A key fluorescent tag for multicolor flow cytometry panels and high-resolution cellular imaging.
• Diagnostic Assays: Integration into lateral flow assays, ELISA, and other immunoassays to enhance sensitivity and detection limits.
• Nucleic Acid Labeling: Staining of DNA and RNA for gel electrophoresis, microarray analysis, and fluorescence in situ hybridization (FISH).
• Material Science: Incorporation into nanoparticles and polymers to create functional, fluorescent materials for sensing and tracking.

Basic Information

Product Name	Cyanine 5 Bihexanoic Acid Dye, Potassium Salt
CAS No.	252255-40-6
Molecular Formula	C37H45KN2O4
Molecular Weight	620.87 g/mol
Synonyms	Cy5 Bihexanoic Acid Potassium Salt; Cy5 Bihex Potassium Salt; Sulfo-Cyanine5 Bihexanoic Acid Potassium Salt; Potassium 6,6'-((1E,3E)-2-((1E,3E)-5-(1-(6-carboxyhexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)cyclohex-2-en-1-ylidene)bis(1-hexyl-3,3-dimethyl-3H-indol-1-ium-5-carboxylate); Cy5 Bihex COOH Potassium Salt; NIR-664 Dye Potassium Salt; Cy5 Bihexanoate Potassium Salt
EINECS	Contact for details

Quality Control

Every batch of Cyanine 5 Bihexanoic Acid Dye, Potassium Salt is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure product integrity, including HPLC purity, absorbance/fluorescence verification, and identity confirmation. A Certificate of Analysis (COA) detailing batch-specific results is available for all orders, supporting compliance in GMP and ISO 13485 environments where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.