Description

5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in biochemistry and molecular biology. This compound is critical for creating stable, fluorescently tagged biomolecules due to its succinimidyl ester group, which efficiently conjugates to primary amines under mild conditions. It is an essential tool for researchers and manufacturers in the fields of diagnostics, flow cytometry, fluorescence microscopy, and nucleic acid analysis, enabling sensitive detection and quantification.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation for use in immunoassays (ELISA, Western blot), immunohistochemistry, and cell imaging.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Flow Cytometry & Cell Sorting: Preparation of fluorescent markers for cell surface and intracellular antigen detection.
• Microarray and Biochip Fabrication: Immobilization of biomolecules on solid surfaces for high-throughput screening.
• Drug Discovery & High-Content Screening: Tagging of target molecules in assay development and pharmacological studies.
• Bioconjugation & Chemical Biology: A key building block for synthesizing advanced fluorescent probes and sensor molecules.

Basic Information

Product Name	5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester
CAS No.	246256-50-8
Molecular Formula	C32H31N3O7
Molecular Weight	569.61 g/mol
Synonyms	5(6)-TAMRA, SE; 5/6-Carboxy-tetramethylrhodamine succinimidyl ester; TAMRA, SE isomer mixture; 6-TAMRA NHS ester; Tetramethylrhodamine-5(6)-isothiocyanate isomer mixture (related); X-rhodamine NHS ester; Rhodamine derivative NHS ester; 5-Carboxytetramethylrhodamine N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and isomer ratio, NMR for structural confirmation, and mass spectrometry. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Isomer Ratio (5-/6-)	Approx. 1:1 mixture
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.