Description

Phen Green SK Diacetate is a cell-permeable, non-fluorescent dye that is hydrolyzed by intracellular esterases to yield the fluorescent Phen Green SK product, a selective fluorescent indicator for labile iron. This compound is critical for researchers and industrial scientists requiring precise detection and quantification of redox-active iron pools within live cells. It is primarily utilized in life science research, pharmaceutical development, and diagnostic assay manufacturing for applications in cellular metabolism studies and metal toxicity screening.

Application

• Live-Cell Imaging: For the real-time visualization and quantification of labile iron (Fe2+) in cytosol and organelles.
• Pharmaceutical Screening: Used in high-throughput assays to evaluate drug candidates that affect cellular iron metabolism or chelation.
• Neurodegenerative Disease Research: Applied in studies of iron dyshomeostasis associated with conditions like Parkinson's and Alzheimer's disease.
• Toxicology Studies: To assess cellular oxidative stress and metal toxicity in environmental and industrial safety testing.
• Diagnostic Kit Development: Serves as a key component in the formulation of kits for research and clinical diagnostic purposes.
• Plant Biology Research: For investigating iron uptake, transport, and homeostasis in plant cells and tissues.

Basic Information

Product Name	Phen Green SK Diacetate
CAS No.	234075-45-7
Molecular Formula	C34H28N2O12
Molecular Weight	656.59 g/mol
Synonyms	Phen Green™ SK Diacetate; Phen Green SK, AM ester; 2,7-Dichloro-3,6-bis(carboxymethylamino)fluorescein diacetate; 3',6'-Bis(carboxymethylamino)-2',7'-dichlorofluorescein diacetate; Iron Sensor Phen Green SK Diacetate; Fe2+ indicator, cell-permeable; PGSD; Phen Green SK DA
EINECS	Contact for details

Quality Control

Our Phen Green SK Diacetate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic verification. Certificates of Analysis (COA) detailing identity, purity, and performance data are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. The product is strictly light-sensitive and must be protected from all light exposure during handling and storage to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~ 490/517 nm (upon hydrolysis)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.