Description

2',3',5'-Tri-o-(T-Butyldimethylsilyl)-4'-C-Hydroxymethyl Uridine is a key protected nucleoside derivative, specifically designed for advanced oligonucleotide synthesis. This compound is critical for researchers and manufacturers requiring precise control over nucleic acid structure and function. It serves as a vital building block in the development of therapeutic oligonucleotides, including antisense drugs, siRNA, and aptamers, for the pharmaceutical and biotechnology industries.

Application

• Oligonucleotide Synthesis: A crucial phosphoramidite precursor for the solid-phase synthesis of modified RNA and DNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for targeted gene silencing therapies.
• siRNA and miRNA Synthesis: Enables the incorporation of specific modifications to enhance the stability, delivery, and efficacy of small interfering RNA (siRNA) and microRNA (miRNA) constructs.
• Aptamer Production: Facilitates the creation of chemically modified aptamers with improved binding affinity and nuclease resistance for diagnostic and therapeutic applications.
• Nucleic Acid Research: Employed in academic and industrial R&D for studying nucleic acid chemistry, structure-activity relationships, and developing novel bioconjugates.
• Prodrug Strategies: Serves as an intermediate in prodrug approaches for nucleotide analogs, aiming to improve pharmacokinetic properties.

Basic Information

Product Name	2',3',5'-Tri-o-(T-Butyldimethylsilyl)-4'-C-Hydroxymethyl Uridine
CAS No.	232588-97-5
Molecular Formula	C30H60N2O7Si3
Molecular Weight	645.09 g/mol
Synonyms	4'-C-Hydroxymethyl-2',3',5'-tri-O-(tert-butyldimethylsilyl)uridine; 2',3',5'-Tri-O-TBDMS-4'-C-hydroxymethyluridine; 4'-(Hydroxymethyl)-2',3',5'-tri-O-[(1,1-dimethylethyl)dimethylsilyl]uridine; Uridine, 4'-C-(hydroxymethyl)-2',3',5'-tris-O-[(1,1-dimethylethyl)dimethylsilyl]-; TBDMS-protected 4'-C-hydroxymethyl uridine; 4'-C-Methylenehydroxy uridine TBDMS ether
EINECS	Contact for details

Quality Control

Our production of 2',3',5'-Tri-o-(T-Butyldimethylsilyl)-4'-C-Hydroxymethyl Uridine adheres to stringent quality protocols suitable for pharmaceutical intermediate applications. Each batch is characterized and tested using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under an inert atmosphere or in a controlled, dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.