Description

4,5-Disulfo Rhodamine-123 Carboxylic Acid Succinimidyl Ester Lithium Salt is a highly reactive, water-soluble fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This compound matters for researchers and manufacturers who require enhanced aqueous solubility and photostability in their fluorescent probes, overcoming limitations of traditional rhodamine esters. It is primarily needed by professionals in bioconjugation, flow cytometry, and advanced microscopy for labeling proteins, antibodies, and other amine-containing substrates.

Application

• Fluorescent Labeling of Proteins and Antibodies: Covalent attachment for immunofluorescence, Western blotting, and ELISA applications.
• Flow Cytometry & Cell Sorting (FACS): Preparation of fluorescently-tagged antibodies and other probes for cell surface and intracellular marker analysis.
• Fluorescence Microscopy & Imaging: Creation of specific, bright probes for confocal, super-resolution, and live-cell imaging studies.
• Bioconjugation & Probe Development: Synthesis of custom fluorescent derivatives for diagnostic assays and research kits.
• Nucleic Acid Labeling: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and related techniques.
• Sensor and Diagnostic Device Fabrication: Integration into biosensors and lateral flow assays requiring stable, water-compatible fluorophores.

Basic Information

Product Name	4,5-Disulfo Rhodamine-123 Carboxylic Acid Succinimidyl Ester Lithium Salt
CAS No.	222164-96-7
Molecular Formula	C34H28Li2N4O14S2
Molecular Weight	806.67 g/mol (Lithium Salt Form)
Synonyms	4,5-Disulfo Rhodamine 123 NHS Ester Lithium Salt; 6-(N-(7-Nitrobenzofurazan-4-yl)amino)hexanoic Acid Succinimidyl Ester (Disulfo Rhodamine-123 derivative); Sulforhodamine 123 NHS Ester, Disulfonated; 5(6)-Carboxy-X-rhodamine N-succinimidyl ester, disulfo; Rhodamine-123 Carboxylic Acid, Succinimidyl Ester, 4,5-Disulfo, Dilithium Salt; Water-soluble Rhodamine 123 NHS Ester; Fluorescent Labeling Reagent, Sulfo-R123 SE
EINECS	Contact for details

Quality Control

Our 4,5-Disulfo Rhodamine-123 Carboxylic Acid Succinimidyl Ester Lithium Salt is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency verification, and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to ensure batch-to-batch consistency and performance reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Labeling Efficiency	≥ 80% (Functional test)
Solubility	Freely soluble in water, DMSO
Absorption Max (λmax)	~505 nm
Emission Max (λem)	~525 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.